Study On The Traditional Chinese Medicine Constitution Characteristics Of Pelvic Organ Prolapse And The Differences Between SOD、 MDA And TGF-betal In Serum

ObjectiveTo study the characteristics of the age and the traditional chinese medicine (TCM) constitution of the patiens with pelvic organ prolapse (POP) and to analysis the relationship between constitution factors,the degree of prolapse and super oxide dismutase (SOD), malondialdehyde (MDA),transforming growth factor-betal (TGF-betal) in serum,in order to offer new ideas for the treatment and prevention for pelvic organ prolapse.Methods1Clinical study:The data came from a survey on TCM constitution of100cases of patients who were diagnosised of pelvic organ prolapse in Fujian Province People Hospital’s outpatient and hospitalization.The survey of TCM constitution was performed by standardized constitution in Chinese medicine questionnaire,to identify the patients’ constitutional types and to analysis the characteristics of the age and TCM constitution of the patiens with pelvic organ prolapse.2Experimental study:Collecting the fasting venous blood of the POP patients with5ml, placing at room temperature for2hours,and using a centrifuge to separate serum from blood.Using the enzyme-linked immunosorbent assay (Elisa) method to determine the concentration of SOD,MDA,TGF-betal in serum.Analysising the differences and correlation.Result1Clinical study(1)The POP patients’age were mainly more than50years old (90%).There were significant differences in the constituent ratios of different ages (P<0.05).There were significant differences between under the age of50and over the age of50(P<0.05).The constituent ratios of different ages over50years old had no obvious difference (P>0.05).(2)The age distributions of POP patients with different degrees of prolapse had significant differences (P<0.05).Prolapse degree was positively related with age and the correlation coefficient was0.318(P<0.05). (3)There were significant differences between single constitution and compound composition in the POP patients (P＜0.05).The constitutions of9kinds of TCM constitution had significant differences (P<0.05).(4)There were significant differences in the distribution of TCM constitution with different age (P<0.05).There were significant differences in the distribution of TCM constitution between under the age of50and over the age of50(P＜0.05).(5)The distributions of TCM constitution of the POP patients with different degree of prolapse had no obvious difference (P>0.05).2Experimental study(1)SOD and MDA in serum were negatively correlated,and the correlation coefficient was-0.180(P<0.05).SOD and TGF-betal in serum were positively correlated,and the correlation coefficient was0.445(P<0.05).MDA and TGF-betal in serum were positively correlated,and the correlation coefficient was-0.275(P<0.05).(2)The contents of SOD and TGF-betal in serum with gentleness type were higher than that of biased constitutional types,and the content of MDA in serum with gentleness type was lower than that of biased constitutional types.SOD in serum with different TCM constitution types had no obvious difference (P>0.05).There were significant differences in MDA,TGF-betal in serum with different TCM constitution types (P＜0.05).(3)There were significant differences in the content of SOD,MDA,TGF-betal in serum with different degrees of prolapse (P＜O.05).(4)The content of SOD and TGF-betal in serum were negatively related with degree of prolapse,and the correlation coefficients were-0.502and-0.642(P<0.05).The content of MDA in serum was positively related with degree of prolapse,and the correlation coefficient was0.697(P＜O.05).Conclusion(1)The POP patients were mainly elderly female (50or higher).Older you get,the more serious the prolapse.(2)Older the POP patiens get,the more obvious the biased constitution tends.The most common biased constitutional type was qi-deficiency type, and it often went together with other TCM constitution types. (3)There must be certain relevance between the degrees of prolapse and SOD, MDA,TGF-betal in serum.(4)The differences between TCM constitution and SOD, MDA, TGF-betal in serum were not clear,which needed further study.