| Objective: To establish rats osteoarthritis animals caused by sodiumiodide and to investigate the influence on extracorporeal shock wave therapytreating rats osteoarthritis sodium iodide, and then reveal the mechanism ofcartilage degeneration mechanical stimulation of extracorporeal shock wavetherapy.Methods:1. Experiment for Model building:8-week-old male Wistar rats were30,these rats were randomly divided into five groups, Group A: Osteoarthritisuntreated group;Group B: two weeks after modeling to receive extracorporealshock wave therapy; Group C: four weeks after modeling to receiveextracorporeal shock wave therapy; Group D: six weeks after modeling toreceive extracorporeal shock wave therapy; Groups E: the control group. A, B,C, D groups in anesthetized by intraperitoneal injection of chloral hydrate, inthe left knee articular injection of sodium iodide solution50ul (iodine sodiumacetate0.2mg, dissolved in saline50ul), the right of none. Group E with thesame method on the left side of the injection of saline. Giving a normal dietlater.2. Extracorporeal Shock Wave intervention: B, C, D groupsintraperitoneal injection of10%chloral hydrate anesthesia, receivingextracorporeal shock wave treatment on the left tibia segment, the energyintensity of the shock wave is set to1.5×105Pa, pulse600times. Treatmentcycle was once a month.3. Using CTX-II test kit to detect the content of rat urine CTX-II’s. Eachmeasurement is normalized total urine creatinine (nmol/l).4. Morphological observation and Mankin score. After the animals werekilled,get the knee joint and fixed in4%paraformaldehyde solution, with10% ethylene diamine tetra-acetic acid decalcified, decalcification was changedonce a week, a total of three weeks. After decalcification is complete, removethe specimens embedded in paraffin. Cut coronal section (5μm thick) andstaining(HE, toluidine blue, safranin-O). According to the Mankin methodfor histologic evaluation method, put the specimen under light microscopy toobserve its detailed structure.5. Using double antibody immunohistochemistry sandwich ABC-ELISAfor detection serum levels of MMP-1, MMP-13, COX-2, IL-1.Results:1. Successful animal model validation：Standard rearing four weeks later,all20rats were sacrificed, the interception of knee surgery done toluidine bluestaining and HE staining，seen in the control group articular cartilage surfaceis smooth, clear hierarchy of chondrocytes, cells were small and flat surface,increasing downward and columnar cells arranged in rare cell division. MIAmade to see if the module is not smooth cartilage surface, cartilage thinningsurface, rough, the surface of the plurality of cartilage fissures, cracks visibleto assigned emitting layer, visible cracks and loose connective tissue sparsemesh connection extending from a surface around the fracture dehydrationcondensation visible necrosis of cartilage cells. Test the left lower limbloading ratio (%); the control group,28.54±8.69; MIA-made modules,49.78±4.08. The results showed that a rat model of knee OA successful model.2. Start measuring1week after ESWT, and found that with the increaseof ESWT time, lower limb strength of B C D group gradually restore. Leftlimb bearing ratio (%):A，23.08±9.37；B，45.37±3.20；C，36.45±5.45；D，29.56±7.29；E，50.62±2.08. The power of group B recoverd best, close tonormal control group E. Group D restored worst, the effect is not obvious.Group C recoverd moderately.3. Compared with group E, the experimental group A, B, C, D in rat urineC-terminal peptide of type II collagen (CTX-II) levels elevated,group A（188.473±13.584pg/ml）was the hightest. Compared with the group E, afterextracorporeal shock wave treatment, the experimental groups B, C, D groups CTX-II levels were reduced. Group B（73.153±9.021pg/ml）accepted ESWTfrom the early, type II collagen C telopeptide (CTX-II) was reduced obviously,gradually approaching the level of content in the control group E（66.150±8.719pg/ml）. C, D groups of (CTX-II) were higher than the normalcontrol group E, and CTX-II level in group D (159.395±14.738pg/ml) washigher than group C(125.262±9.522pg/ml).4. Compared with normal control group (Group E), Group A’s Mankinscore results in a significant increase in Group D, Group D osteoarthritisdisease turn for the better. However, Group B, Group D began to accept earlyextracorporeal shock wave therapy, osteoarthritis condition improved, tends tonormal levels.5. Compared with the control group, MMP-1in serum of Group A（78.32±2.34ug/ml）was significantly increased by about3.5times. MMP-13in the group B serological levels （20.61±1.13ug/ml）similar to t normalcontrol group. Compared with normal control group E, C group and D groupin serum level of MMP-13respectively about23times.6. Compared with the control group (E), the MMP-1levels of group A(178.03±3.20ug/ml) were significantly increased, an increase of2.8times.MMP1in group B serological levels (72.60to1.15ug/ml) and normal controlgroup are similar, returns to normal levels. Serum levels of MMP-1in GroupC (116.48±2.66ug/ml)approximately increased by1.7times, Group D(162.48±2.18ug/ml）by2.3times. Thus, ESWT inhibits MMP1level; Alongwith the extension of ESWT time, MMP1serum levels reduced.7. Compared with the control group (E), serum cox-2levels of Group Aincreased significantly. Serum cox-2levels of Group B tended to be normal;Group C was about2times of Group E; Group D was about3.2times of groupE8. Compared with the control group (E), serum cox-2levels of Group A（19.32±0.064pg/ml）increased significantly, was about3.5times of group E; Serum cox-2levels of Group B（6.46±0.057pg/ml）is similar to normalcontrol group level; Serum cox-2levels of Group C（10.25±0.072pg/ml）is about1.8times of Group E（5.93±0.031pg/ml）; Serum cox-2levels of GroupD（10.25±0.072pg/ml）is about2.5times of Group E.Conclusions: Intra-articular injection of sodium iodide is a reliablemethod for the production model of osteoarthritis. Extracorporeal shock wavetherapy on sodium iodide-induced rat osteoarthritis have a better therapeuticeffect. The sooner the better to accept extracorporeal shock wave therapy. |
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