The Association Of The ERCC2/XPD Gene Polymorphism With The Risk Of Colorectal Cancer

Objective:To Discuss the relationship between ERCC2/XPD geners13181G/T and rs1799793A/G polymorphism and genetic susceptibility tocolorectal cancer, providing a theoretical basis for the diagnosis and treatmentof colorectal cancer.Methods:Two SNPs of ERCC2/XPD gene rs13181G/T andrs1799793A/G patients with211patients with CRC from Tumor Hospital ofHebei Province and132healthy controls were analyzed using polymerasechain reaction-ligase detection reaction (PCR-LDR) method. Frequency ofhaplotypes and linkage disequilibrium of ERCC2/XPD gene in differentgroups were analyzed by EH and2LD programs. The ratio is calculated usinglogistic regression relative risk ratio (odds ratio, OR) and95%confidenceintervals (confidence interval, CI).Results：1Application of the law of Hardy-weinberg to test the genotypefrequencies in the control population, the results P>0.05, indicated that thepopulation is representative.2The frequency of G and T of ERCC2/XPD rs13181in rectal cancerpatients and the control group were31.11%、68.89%and17.8%、2.2%.Therewas significant difference between the two groups(P<0.05).The frequency ofG and T of ERCC2/XPD rs13181in colon cancer patients and the controlgroup were28.68%、71.32%and17.8%、82.2%.There was significantdifference between the two groups(P=0.02). The genotype frequency of GG,GT, and TT in Colon cancer patients and control group were6%、23.48%、70.45%and16.18%、25.00%、58.82%,the GG increased the risk of coloncancer,(OR=3.179,95%CI=1.196-8.546).The genotype frequency of GG,GT, and TT in rectal cancer patients and control group were18.18%、25.87%、 55.94%and6%、23.48%、70.45%,it was significant(P<0.05).Compared withthe TT genotype, the GT and GG genotype increased the risk of rectalcancer.(OR=2.72，95%CI=1.08-6.86and OR=3.77，95%CI=1.62-8.81).3The frequency of G and A of ERCC2/XPD rs1799793A/G in coloncancer patients and the control group were16.2%、83.8%and15.15%、84.85%.There was no significant difference between the twogroups(P>0.05).The frequency of G and A of ERCC2/XPD rs1799793A/G inrectal cancer patients and the control group were17.83%、82.17%and15.15%、84.85%.There was no significant difference between the twogroups(P>0.05). The genotype frequency of GG and GA in Colon cancerpatients、control group and rectal cancer patients were67.65%32.35%and69.7%、30.3%及64.34%、35.66%,it has no significant(P>0.05).There was nosignificant association between the GA and GG genotype of colorectal cancerpatients and control group (OR=0.784,95%CI=,0.473~1.299and OR=0.909,95%CI=0.485~1.706).4In colon cancer patients,there was linkage disequilibrium betweenrs13181G/T and rs1799793A/G（D’=0.492).The most frequent haplotype wasrs13181T-rs1799793G (69.7%);The haplotype of rs13181G-rs1799793G couldincrease the incidence of colon cancer（OR=1.421，95%CI=1.210-3.954).Thehaplotype of rs13181G-rs1799793G was opposite （OR=0.523,95％CI=0.362-0.941);The haplotype of rs13181T-rs1799793A was unrelated to thedisease.In rectal cancer patients,it was the same to the colon cancerpatients,there was linkage disequilibrium between rs13181G/T andrs1799793A/G （D’=0.021).The haplotype of rs13181T-rs1799793G couldreduse the incidence of colon cancer（OR=0.523,95％CI=0.354-0.972）.Thehaplotypes of rs13181T-rs1799793A and rs13181G-rs1799793G was opposite（OR=1.783，95%CI=1.060-2.997)及（OR=1.231，95%CI=1.423-2.8).Conclusions:1The gene polymorphism of ERCC2/XPD rs13181G/T may beassociated to colorectal cancer in the Hebei populations. related to increasedrisk of colon cancer Compared with the TT genotype,the GG genotype increased the the risk of colon cancer, the GT and GG genotype increased therisk of rectal cancer.2It may has no significant correlation between ERCC2/XPD geners1799793and the colorectal cancer in the Hebei populations.3In colorectal cancer patients,there was linkage disequilibrium betweenrs13181G/T and rs1799793A/G.