Anthricin, Extracts And Derivatives And The Antitumor Activity Of Bcl-2/Bax Effects And Cell Cycle And Apoptosis Were Observed

Objective:Discussion5-(3-methoxy-1-propenyl)-1,3-benzodioxan metallocene (13) and picrop odophyllin (15) on HepG2, A549, Hela and MG-63tumor cells in vitro anti-tumor a ctivity; investigate Anthriscus lactone, on the13th, the15th and4’-demethyl Anthrisc us lactone (No.0derivatives) for Bcl-2, Bax role; explore Anthriscus lactone,13,15, No.0derivatives on four tumor cell cycle and apoptosis.Methods:MTT assay using different concentrations (0,5,10,20,40,80,160ug/ml) on the13th, the15th of HepG2, A549, Hela and MG-63cell proliferation; detected using a microplate reader Anthriscus lactone,13,15,0derivatives of HepG2, A549, Hela and MG-63tumor cells Bcl-2, Bax effect observed in Anthriscus lactone,13,15,0derivatives as observation group, paclitaxel as a positive control group to0.1%DMSO as a control group, the changes observed for48h after Bcl-2and Bax; using flow cytometry Propidium Iodide (PI) single standard detection cell cycle progression and apoptosis impact to Anthriscus lactone,13,15,0derivatives as the observation group, paclitaxel as a positive control group to0.1%DMSO as a control group.Results:1.13for the A549,HepG2,MG-63,HeLa four tumor cell proliferation were signifi cantly inhibited with IC50values of403.023μg/ml,83.893μg/ml,274.191μg/ml,95.086μg/ml; the15th to the A549, HepG2, MG-63, HeLa four tumor cell proliferation w ere significantly inhibited with IC50values of41.912μg/ml,81.574μg/ml,72.840μg/ml,16.582μg/ml.And with the extension of the drug concentration and time, the inhib ition rate is higher, so a time-a dose-dependent manner.2. by microplate testing showed that paclitaxel group compared with the control group, the role of the four tumor cells48h Bcl-2/Bax ratio was significantly decre ased, and there was a significant difference (P<0.05); Anthriscus lactone group and t he blank the control group, the role of the four tumor cells48h Bcl-2/Bax ratio w as significantly decreased, and there was a significant difference (P<0.05); the13th compared with the control group, only the role of Hela, MG-63cells after48h Bcl-2/Bax ratio was significantly decreased, and there was a significant difference (P<0.05);15with the control group role HepG2, MG-63and A549cells after48h Bcl-2/Bax ratio was significantly decreased, and there was a significant difference (P<0.05);0derivatives group and control group after the role of four tumor cells48h Bcl-2/Bax ratio was significantly decreased, and there was a significant difference (P<0.05).3. by flow cytometry with PI standard test Anthriscus lactone,13,15and0deriv atives of cell cycle progression and apoptosis show that with the increase of the rol e of time, the percentage of cells in G0/G1phase decreased, G2/M phase of the c ell percentages changed little, while the gradual increase in the percentage of cells i n S phase, the description Anthriscus lactone,13,15,0block of four derivatives of the tumor cells in the S phase, and cell sub-Gl cell subsets apoptotic peak incr eases with the increase of time and therefore description Anthriscus lactone,13,15,0, four derivatives induce tumor cell apoptosis.Conclusions:The13th and the15th of the A549, Hela, HepG2and MG-63tumor cells signif icantly inhibited the proliferation and the role of time-was a certain relationship bet ween drug concentration; Anthriscus lactone,13,15and0derivatives through increas e Bax, down Bcl-2, to promote apoptosis of tumor cells; Anthriscus lactone,13,15and0derivatives induce apoptosis in cancer cells, but can also make changes in cell cycle progression.