The Interaction Between JAK/STAT And PI3K/AKT Signaling Pathway And Their Effects On Cerebral Ischemia Reperfusion Injury In Rats

Object:To investigate the interaction between the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signal pathway and phosphoinositide-3-kinase/set-inethreonine kinase(PI3K/AKT) signal pathways, as well as their effects on cerebral ischemia reperfusion injury (I/R) injury in rats.Patients and methods:Control group:Saline is injected intraperitoneally20min before the preparation of the middle cerebral artery ischemia (MCAO) model. After90min of ischemia, reperfusion24h. Then evaluate the neurological deficits, measure the infarct by TTC staining, and detect the expression of P-JAK2、P-AKT protein in brain. AG group: AG490is injected intraperitoneally20min before the MCAO, then do the same operation as control group. WM group:Wortmannin is injected intraperitoneally20min before the MCAO, then do the same operation as control group. Sham group:Same as the control group, except for the ischemia. Results:1. The rats of sham group did not appear neurological deficits, the remaining rats in each groups appeared different degree of neurological deficits. Compared with control group,AG490group score significantly lower,there were significant differences(P<0.05)2. No infarction was observed in the sham group, but obviously developed in the other groups. Compared with control group, infarct volume increased in wortmannin group and decreased in AG490group, the differences were both statistically significance (p<0.05).3.Rat’s brain existed based secretion of P-JAK2and P-AKT, after I/R, the P-JAK2and P-AKT increased. Compared with the control group, after using Ag490, P-JAK2decreased obviously, but P-AKT increased significantly. After using wortmannin, P-AKT decreased significantly, while P-JAK2had no significant changes.Conclusion:The activation of PI3K/AKT signaling pathway can effectively reduce I/R injury. The activation of JAK/STAT signaling pathway will increase the injury. JAK/STAT signaling pathway can have an impact on the PI3K/AKT pathway, AG490by inhibiting JAK/STAT signaling pathway, increasing p-akt in I/R brain, play a role in brain protection.