Mir-126Regulates Cell Invasion Through Expression Of VEGF In Ovarian Cancer

BackgroundOvarian cancer is one of the most popular gynaecologic oncology. Due to deficiency of early symptoms, most cases are diagnosed at an advanced stage. Ovarian cancer has a strong malignant behavior, and it is prone to planting in Abdomen. Ovarian cancer has been a threat against women’s life. MicroRNAs are non-coding RNAs in eukaryocytes, they have been shown to play crucial roles in diverse biological processes, such as cell development, signal transduction, tissue dif-ferentiation and maintenance, disease, and carcinogenesis. MiR-126is the only miRNA known to be specifically expressed in endothelial cell lineage, hematopoietic progenitor cells, and endothelial cell lines. It is able to regulate the expression of VEGF and other genes involved in the regulation of vascular development, angiogenesis and vascular inflammation and other vascular physiology and pathophysiology. Studies have shown that miR-126can inhibit the proliferation and metastasis of various tumors, such as lung cancer, breast cancer, colon cancer, suggesting that miR-126may have a potential role of tumor suppressor genes. Tumor angiogenesis is closely related to tumor development. VEGF is a specific angiogenic factor, which has a strong promote tumor angiogenesis capabilities. Researches have shown that miR-126can inhibit the expression of VEGF. Our study, by means of culturing SKOV3ovarian cancer cells in vitro, and transfected with anti-miR-126gene, we attempt to reveal the role of VEGF and miR-126in ovarian cancer invasion and metastasis.Materials and MethodsThe ovarian cancer cell lines used in this study was SKOV3, transfected with anti-miR-126. The control team includes LV3NC, LV3-has-miR-126inhibitor or LV3-has-miR-126transfected cells. Flow cytometry was used to detect the cell cycle, Transwell cell invasion experiment is carried out to observe the invasion ability of each group, then to analyze the relationship of expression of miR-126and ovarian cancer cell invasion ability. And by immunofluorescence, Western Blot, we compared the difference of VEGF expression in each group, analysed the relationship of miR-126and VEGF.Results1. The proportion of cells in S period was63.9in SKOV3group,53.1in SKOV3/LV3NC group, and34.7in SKOV3/LV3-has-miR-126group respectively. Amid the groups, the proportion of cells in Glperiod was higher but lower in S period in SKOV3/LV3-has-miR-126group. The results revealed the up regulation of miR-126may inhibit cell synthesis in S period.2.248±16cells can invade the cell membrane in the SKOV3group,250±15(P>0.05) in the controlling team, and169±12(P<0.05) in the LV3-has-miR-126group amid the cell invasion team. 3. The proportion of VEGF protein expression was almost the same comparing the SKOV3group to the SKOV3/LV3NC group, the relative proportion was71.3±2.7and70.6±2.3. However, it was32.4±4.2in the LV3-has-miR-126group, which was significantly lower than the other groups.Conclusion1. The transfer of miR-126was able to inhibit cell synthesis in S period but increase cells in G1period. Meanwhile, the transfer decreased cells which can invade cell membrane. The results give a hint that expression of miR-126play a role in proliferation and invasion of ovarian cancer.2. Expression of VEGF was decreased in cells transferred with miR-126, which revealed expression of VEGF may related to the effect.