The Role Of Notch Signaling Pathway In Nerve Regeneration

Objective： To Observe the role of Notch signaling pathways in adult nerve regeneration.Method: Male mice of thirty five C57/BL were randomly divided into ischemia group,DAPT group （indicating ischemia and DAPT）and sham group. After middle cerebralocclusion(MCAO),experimental mice group were randomly injected DAPT and DMSO(100mg/kg）by intraperitoneal injection. Ten days after MCAO, the mices were injectedBrdU (50mg/kg）for three consecutive days. After12hours, sacrificing the mices，thehippocampus were dyed with GFAP/BrdU, DCX/BrdU immunofluorescence doublestandard to observe the changes of cells.Western Blot had test the change of the Notch1signaling proteins.The Real time PCR had detected Hes1relative expression ofmRNA.Result：After MCAO,among ischemia group, DAPT group （indicating ischemia andDAPT） and sham group,GFAP/BrdU, DCX/BrdU positive cells of ischemia group wassignificantly increased (P <0.01).Western Blot results suggest that ischemia can promoteNICD protein expression (P <0.01), DAPT can decrease the NICD protein expression (P<0.01).Real time PCR results indicate that the Hes1mRNA relative expression ofhippocampal tissue were significantly increased in ischemia group (P <0.01).Ischemia canpromote Hes1mRNA expression,but DAPT may reduce Hes1mRNA expression.,Conclusion: After cerebral ischemia Notch signaling is involved in the differentiation ofneural stem cells.