Effects And Mechanisms Of Remote Ischemic Preconditioning In The Ischemia-reperfusion Injury In Rats With Non-alcoholic Fatty Liver

Objective To investigate the protective effect of remote ischemic preconditioningagainst liver ischemia/reperfusion (I/R) injury in rats with fatty liver and their possiblemechanisms.Methods The model of fatty rat was induced by feeding high-fat diet. Subsequently,the fatty rats were randomly divided into four groups：sham group(S group), hepatic I/Rgroup, RIPC group, RIPC+hepatic I/R group, with8rats in each group. The hepatic I/Rmodel was produced by occlusion of hepatic inflow for40min and then reperfusion for2h,RIPC was induced by6cycles of5min occlusion and5min reperfusion of the unilateralhind limb using a tourniquet, and RIPC+I/R was elicited by6cycles of5min occlusionand5min reperfusion of the unilateral hind limb using a tourniquet followed by the I/R.All ischemia rats were only subjected to70%liver ischemia. Finally, blood and liversamples were obtained to determine the activity of ALT, AST, the expressions of HSP70protein, and pathology.Results Compared with S group and RIPC group, all other groups had higher levelsof AST and ALT (P＜0.05)，while these two indices had no significant differences betweenS group and RIPC group (P＞0.05)，but those in RIPC+I/R group were obviously lowerthan that in I/R group(P＜0.05). Compared with S group and RIPC group, all other groupspresented hydrops, degeneration and inflammatory cell infiltration of varying degrees onthe basis of fatty degeneration, but the pathological changes in RIPC+I/R group wereobviously milder than those in I/R group. Compared with S group, the HSP70expressionsin liver tissues of other groups all significantly increased (all P<0.05), but the HSP70 expression in RIPC+I/R group were significantly higher than that in I/R group (P<0.05).Conclusion RIPC has protective effect against I/R injury of the hepatic adiposeinfiltration. The protective effects possibly are due to induction of protein HSP70expression.