| Objective:Cadherins and catenins play an important role in mediating intercellular adhesion and maintaining vascular permeability. On the one hand, cell adhesion is of vital importance in development of tumor. Invasion and metastasis of tumor are usually accompanied by the reduce of intercellular adhesion strength. Cadherins and catenins are two main types of intercellular adhesion factors which constitute complex adhesive connections and mediate adhesion between the cells. On the other hand, the changes of the intercellular adhesion factors and adhesive connection compound will cause the change of vascular permeability which is one of the important physiological and pathological changes of many diseases and inflammation. In order to study the expression of cadherins and catenins in ovariectomized (Ovx) rats after treated with17β-estradiol, we make use of ovarian rats as a model and use tamoxifen, sunitinib, resveratrol and genistein to explore whether the effects of17β-estradiol on cadherin and catenin expressions are related to estrogen receptors and tyrosine kinase signal transduction pathways.Methods:Divided54SD female rats into nine groups with random grouping, and each group had six rats. All the groups included Sham group, Ovx group, Ovx+Est0.01group, Ovx+Est0.1group, Ovx+Est1.0group, Ovx+EstO.l+Tam group, Ovx+Est1.0+Sut group, Ovx+Est1.0+Res10.0gro-up and Ovx+Est1.0+Gen10.0group. Ovx rats were subcutaneously given17β-estradiol,17β-estradiol combined with tamoxifen, sunitinib, resveratrol or genistein for21days. Taking sham operation rats as a comparison. The expression of E-cadherin, a-catenin and β-catenin was examined by immunohistochemical technique and western blot technique.Results:1. The expression of cadherins and catenins in the rat uterusImmunohistochemical results showed that E-cadherin, a-catenin and P-catenin were expressed in the uterus tissue in each of the experimental group. E-cadherin, a-catenin and β-catenin mainly expressed in the cell membrane of endometrial epithelial cells and glandular epithelial cells and little expressed in the cytoplasm. And β-catenin also expressed in the nucleus of mesenchymal cells and muscle cells.2. The effects of17β-estradiol on the expressions of E-cadherin, a-catenin and β-catenin.After the ovary removal, we found the rat uterus was atrophic. Compared with Ovx group, the expression of E-cadherin, a-catenin and β-catenin were decreased in high and middle dosage17β-estradiol group. And there was no different between the Ovx group and the low dosage17β-estradiol group.3. The effects of selective estrogen receptor modulators on the expressions of E-cadherin, a-catenin and β-catenin induced by17β-estradiol in ovarian uterus of rats.1) Tamoxifen could restrain the down regulation of E-cadherin, a-catenin and β-catenin in the middle dosage estrogen group.2) Resveratrol and genistein could cooperate with the17β-estradiol in decreasing the expression of E-cadherin, a-catenin and β-catenin.4. Sunitinib, as a tyrosine kinase inhibitor, made no obvious effect on the down regulationof E-cadherin, a-catenin and β-catenin induced by17β-estradiol.Conclusion:1. After ovariotomy, the expressions of E-cadherin, a-catenin and β-catenin significantly increase in rat uterus.17β-estradiol could decrease the expression of E-cadherin, a-catenin and β-catenin with no dose-dependent.2. The down regulations of E-cadherin, a-catenin and β-catenin induced by17β-estradiol may be related to estrogen receptor.3. The effects of17β-estradiol on down regulating the expressions of E-cadherin, a-catenin and β-catenin may have no direct relation to the influence of tyrosine kinase signal transduction pathways. |
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