TLR4Expression In Intestinal In Rats With Nonalcoholic Fatty Liver Disease

Objective: To investigate the changes of TLR4expression in intestinal ofthe rats with NAFLD and intervention of Bifidobacterium triple viable capsule.Methods:A total of40adult male SD rats were randomized into4groups:normal control group (group I,N=10),feeding with normal diet，preventiongroup (group II,N=10) feeding with high-fat diet and Bifico gavage frombeginning, therapy model group(group III, N=10) feeding with high-fat diet andBifico gavage after12weeks,high-fat control group (group IV, N=10) feedingwith high-fat diet. Group I and IV were given the same amount of normalsaline.Seventeen weeks later, the rats were killed for having a liver biopsy toconfirm whether NAFLD was successfully induced. The expression of intestinalTLR4was detected by immunohistochemistry and TLR4mRNA by Real-timeFQ-PCR. Results:(1) The expression of TLR4mRNA was significantly higher ingroupII、III、IV, compared with group I (P<0.05)，and lower in groupII、III,compared with group IV. There were no difference between group IIand groupIII.（P>0.05);(2)The levels of group II、III、IV serum ALT、AST、CHO、LDL were significantly higher than that ingroup I.(P<0.05);(3) The liverinflammation degree ofgroup II、III、IV were significantly higher than group I(P<0.05).There were no difference between group II、III、IV;(4)The expressionof TLR4in intestinal epithelial cell (immuneohistochemistry) was significantlyhigher in group II、III、IV, compared with group I and group II、III weresignificantly lower compared with group IV（P<0.05).There were no differencebetween group II and group III.（P>0.05)Conclusions:The expression of TLR4in rats with NAFLD weresignificantly higher than that in normal diet group.Bifico exert a preventive andtherapy effect on NAFLD induced by high-fat diet in rats probably by reducingthe level of Lipopolysaccharides.