Anti-hepatocarcinoma Effect Of Salidroside And Its Mechanism

Objective1.To evaluate the anti-tumor effect of Salidroside on ascitic-fluid typehepatocarcinoma H22in vitro.2.Made the tumor-bearing mice model to study the anti-hepatoma effect ofSalidroside and investigate its mechanism.Methods1.The cytotoxicity of Salidroside on ascitic-fluid type hepatocarcinoma H22was detected by CCK-8assay in vitro.2.The ascitic-fluid type hepatocarcinoma H22was transplanted to mice toevaluate the anti-tumor effects of Salidroside in vivo.3.The Antitumor mechanism of Salidroside was investigated by themethods of ELISA, cytotoxicity assay and agarose gel electrophoresis assay.Results1.Salidroside inhibited proliferation of ascitic-fluid type hepatocarcinomaH22in both time and concentration dependent manners.2.The general situation of the Salidroside mice was better than the NSgroup,Salidroside (20,40,80mg·kg-1) could reduce the ascites at the volume of 5.60±2.17ml,4.90±1.13ml and3.17±1.53ml respectively, while the volume inNS group was6.91±1.38ml，Salidroside (40，80mg·kg-1) were statisticallysignificant compared to NS group（P<0.05）. It also significantly lengthened thesurvival time of H22ascitic-tumor mice with the life extension rate37.54%、48.96%and52.85%respectively. It showed inhibitory effects on H22solidtumor with inhibition ratio28.9%、43.3%and53.5%respectively.After it plussmall dose of CTX（10mg.kg-1）,the inhibitory ratio was35.2%、48.0%and55.9%respectively. These results suggested that Salidroside had significantantitumor effect in vivo.3.Compared with NS group, Salidroside (40，80mg·kg-1) could increase thespleen index,thymus index and the content of serum IL-2and IL-12（P<0.05）.as well as the T lymphocyte Stimulation index and the killing activity of NKCells （P<0.01） on mice bearing H22tumor． The results of agarose gelelectrophoresis indicated that Salidroside （4.0~8.0mg.L-1）could inhibit thecatalytic activity of Topo II on H22tumor cell.ConclusionSalidroside has marked antitumor effect both in vitro and in vivo,itsmechanism is probably related to improving the anti-tumor immune functionand inhibiting the catalytic activity of Topo II.