In Vitro Effects Of Chlorogenic Acid/Isochlorogenic Acid On Aspergillus Fumigatus Biofilm

Objective To investigate the in vitro destructive effect of Chlorogenicacid (CRA)/isochlorogenic acid(IRC) on aspergillus fumigates (A. fumigatus)biofilm.Methods The in vitro biofilm model was established by setting clinicalisolates of A. fumigatus as the study strain minimum inhibitory concentrations(MIC) for antimicrobial agents following a microdilution method, A. fumigatuswere measured by doubling dilution. Scanning electron microscope (SEM) andlaser confocal scanning microscope (CLSM) was used for biofilm qualitation.Crystal violet staining method was used for biofilm quantitation.Results After being reacted with CRA/IRC for24h or48h, the qualitationof biofilm showed that A. fumigatus extracellular matrix was thinner than thecontrol group. The quantitation of biofilm showed that the optical densities were (1.05±0.19),(1.14±0.26),(1.16±0.14) for CRA group,(1.18±0.06),(1.21±0.06),(1.26±0.04) for IRC group in24h, and (1.91±0.17) for the control group(P<0.05). The quantitation of biofilm showed that the optical densities were(2.25±0.05),(2.27±0.05),(2.31±0.03) for CRA group,(2.26±0.02),(2.27±0.02),(2.29±0.04) for IRC group in48h,(2.36±0.01) and (2.36±0.01) for the controlgroup (P<0.05).Conclusion1、we can build aspergillus biofilm model in vitro；2、CRA/IRC can inhibit the biofilm formation of A. fumigatus.