Research Of Serum Lipopolysaccharide Binding Protein And Calcium-binding Protein S100A8before And After Mechanical Ventilation In Thoracic Surgery

Objective To study the changes in expression of serum calcium-bindingprotein S100A8（Calcium binding protein S100A8， S100A8） andlipop-olysaccharide binding protein （LPS-binding protein，LBP）before andafter mechanical ventilation, Understand their relationship withventilator-associated lung injury， Provide experimental basis for furtherunderstanding of the biological mechanisms of VALI.Method （1）20cases of lung cancer patients undergoing thoracotomylobectomy were devided into two-lung ventilation (two-lung ventilation, TLV)group and one-lung ventilation (One lung ventilation, OLV) grou-p atrandomized controlled case study method，10cases in each group.（2）Inclusioncriteria:patients with normal lung function or only mild ventilatory dysfunction;liver and renal function was normal;cardiac fun-ction I-Ⅱlevel.Case collectionprocess required to remove followingthe conditions::pulmonary ventilation process for any reason unable to maintainoxygen saturation above90%, such as:double-lumen endotrac-heal tube pooralignment restrictions and and postural restrictions;OLV time less than2h ormore than4h; bleeding too much, great hemodynamic fluctuations;use of hormones or vasoactive drugs intraoperative; patients with severe hypoxemia inanesthesia resuscitation, can not be successfully extubated.(3)Each patientusing the same method of anesthesia, anest-hesia machine and monitoringequipment, operated by the same anesthesiologist.(4) The central venous blood5ml extracted from each patient in the following four point: before anesthesia(T0), at the end of surgery (T1),24h after surgery (T2) and48h aftersurgery(T3),then placed in a blood coagulant tube, to be coagulated，centrifugedat3000rpm/min speed20min within4℃constant temperature, took theupper serum, stored at-80℃.（5）The S100A8and LBP in serum were testedby immunoblotting (Western Blot, WB).Results There was statistically significant difference about S100A8between the two groups at each time point (P <0.05); The two groups at eachtime point compared with T0，T1, T2, T3point difference was statisticallysignificant (P <0.05)；There was no statistically significant difference about eachtime point of the two groups（P＞0.05）. There was an interaction betweengroups and between the time points，that the two groups differ in themagnitude of elevated S100A8, OLV group had a bigger rise over the TLVgroup.Conclusion S100A8and LBP may be involved in the process of VALI;OLV is still a safe way of ventilation.