Correlation Analysis Of Airway Epithelial Cell Interleukin-25and α-smooth Muscle Actin In Asthmatic Airway Remodeling

Background:Bronchial asthma is caused by various inflammatory cells such as eosinophils (EOS), mast cells, T lymphocytes and cellular components involved in a variety of chronic inflammatory airway disease, and usually the structure of airway epithelium characteristic pathological changes. The three main pathological changes include bronchial asthma:airway inflammation, airway remodeling and smooth muscle disorders, chronic inflammation can cause some release of cytokines and media these inflammatory cytokines and inflammatory mediators through a certain way can cause airway remodeling. Inflammatory cytokine interleukin-25(IL-25) is a member of the IL-17family, which in the initiation, promotion and regulation of Th2-type immune responses play an important role, but found that the peripheral blood and induced sputum of patients with asthma IL-25levels were significantly higher, and also found in the airway epithelium while IL-25expression in airway remodeling associated with asthma early indicators of α-smooth muscle actin (a-SMA) and mucosal airway basement membrane thickness was significantly lower increased. Therefore, airway epithelial IL-25may be associated with airway remodeling in asthma there is a certain link. The experiments done on the relationship between the related research..Purpose:Through inducing asthma model in mice, investigate bronchial asthma airway epithelial cells interleukin-25and α-SMA changes and explore interleukin-25and α-smooth muscle actin remodeling in asthmatic airways relations.Methods:Measured by the asthma group with chicken ovalbumin (OVA) to build an animal model of asthma nebulizer mouse asthma group, atomized saline control group, using histological examination (immunohistochemistry), enzyme immunoassay, Western Blot and other methods control mice serum, bronchoalveolar lavage fluid and lung tissue IL-25expression and a-SMA, using SPSS20.0statistical package for statistical data processing, all data are expressed as mean and standard deviation of measurement data t-test, test level α=0.05, P<0.05was considered statistically significant. Further analysis of IL-25and a-SMA whether there is some connection in bronchial asthma.Results:(1) In this study, we found that by enzyme-linked immunosorbent assay (ELISA) asthma group IL-25and α-SMA expression levels in serum and bronchoalveolar lavage fluid was significantly higher compared with the control group, the difference was statistically significance (P<0.05); Asthma group correlation analysis IL-25and α-SMA positive correlation.(2) By immunohistochemistry IL-25and α-SMA expression in the lung tissue of asthmatic mice, almost no expression in the lung tissue of mice in the control group.(3) Western Blot Detection by IL-25and α-SMA expression in asthmatic mice lung tissue levels are significantly increased, compared with the control group, the difference was statistically significant (P<0.05);Asthma group, IL-25and correlation analysis of a-SMA positive correlation.Conclusion:The bronchus asthma model in serum, alveolar lavage fluid and lung tissue inflammation cytokines IL-25compared with the normal group is significantly higher expression, at the same time α-SMA in bronchial asthma, alveolar lavage fluid and serum in the model is also significantly higher expression in lung tissue, while a-SMA in bronchial submucosal deposition can lead to remodeling of the airway, showed that IL-25and α-SMA in asthmatic airway remodeling resulting in a certain degree of correlation.