Bone Marrow Mesenchymal Stem Cells Transplantation Preconditioned With Exogenous Hydrogen Sulfide In The Treatment Of Rat Myocardial Infarction

Background: Myocardial infarction (MI) is the most serious cardiovascular diseasewith highly mortality. The treatment of MI currently is drug therapy, PercutaneousCoronary Intervention (PCI) and Coronary Artery Bypass Grafting (CABG). Althoughthese methods can alleviate the symptoms of chest pain, improve blood reperfusion,with myocardial infarction patients, cardiac function is difficult to recover and lead toheart failure ultimately. Bone-Marrow Mesenchymal stem cell (BMMSCs) is a kind ofpluripotent adult stem cells. With many advantages of wide variety of sources, easyproliferation, highly gene transfection rate, BMMSCs can be used as a treatment ofmyocardial infarction. The application is limited by the low transplantation survival rate.Hydrogen sulfide (H2S) emerged as a new gaseous signal molecule, widely present inthe human body. It takes an important part in many physiological and pathologicalprocesses. The researches have shown that H2S has the effect of anti-inflammatory,anti-apoptotic.Objective: To study the effect of hydrogen sulfide (H2S) on the BMMSCstransplantation for treatment of myocardial infarction. We use exogenous H2S donorNaHS preconditioned with BMMSCs and evaluate the effect of exogenous H2Spreconditioned BMMSCs transplantation.Methods: BMMSCs were separated and cultivated form Sprague-Dawley rats weighing (100±20) g. The4thgeneration cells were used for later experiment, and marked byDAPI at2hours before use. Fifty male Sprague-Dawley rats, weighing (200±20) g hadbeen divided into five groups: Sham group (n=10) and four transplantation groups:BMSCs(n=10), H2S-BMSCs(n=10), H2S(n=10), normal saline (n=10). The myocardialinfarction (MI) model of four groups had been established execept sham group (onlythread without ligation). The cardiac function was measured by echocardiogram at4weeks after cell transplantation. The collagen in the infarction area was tested byMasson staining, and the infarction size was measured by image analysis software.Results: Severe myocardial fibrosis was found in the normal NS group, with nomyocardial regeneration in the infarct area and large infarction size. H2S-BMSCs grouphad less collagen and less infarction size than BMSCs or H2S groups. Left ventricularejection fraction and left ventricular fractional shortening of the H2S-BMSCs groupwere significantly higher than those of the BMMSCs or H2S groups.Conclusion: The cells survival rate and cardiac function of myocardial infarction ratscan be promoted by H2S-preconditioned BMSCs transplantation, which is superior toBMSCs or H2S alone.