Talin-l Is Correlated With Invasion And Migration In Primary Liver Cancer

AIM: Detection the invasion and migration abilities of different types of liver cancercells and normal human liver cell in vitro as predict malignant progression andprognosis. Investigate the relationship between the mRNA and protein expression ofTalin-1and the ability of invasion and migration in human liver cell lines. Then, Tostudy the level of Talin-1expression is correlated with invasion and migration inhuman hepatocellular carcinoma.METHODS: We cell culture the highly metastatic human liver cancer cell lineMHCC-97H cell line and the minimally metastatic liver cancer cell lines MHCC-97Lcell line and human liver cancer lines such as MHCC-97L cell line, MHCC-97H cellline, SMMC-7721cell line, BEL-7402cell line, HePG-2cell line and normal humanliver cell (LO2cell line), We measured the mRNA and protein expression levels ofTalin-1in five human liver cancer cell lines and normal human liver cell (LO2cellline) by real-time PCR and western blot. The migration and invasion of the cells wereassessed using transwell assays and cell scratch experiments, respectively, andproliferation was assessed by soft AGAR colony formation.RESULTS: Talin-1mRNA expression differed significantly between liver cancer celllines and the normal liver cell line (LO2cell line)(P<0.0001). The MHCC–97L cellline exhibited higher Talin-1expression than LO2cell line (P<0.0001). The Bel-7402and HePG-2cell lines exhibited lower Talin-1mRNA levels than LO2cell line(P<0.0001). The MHCC-97H and SMMC-7721cell lines also exhibited lower Talin-1expression than LO2cell line (P>0.05). The protein expression of Talin-1in the liver cancer cell lines and the normal liver cell line differed significantly. MHCC–97L cellline exhibited elevated Talin-1protein levels (P<0.05).Compared with the LO2cellline, the high Talin-1expressing MHCC-97L cell line exhibited reduced invasioncompared with the other cancer cell lines (P=1.000). The Bel-7402and HepG-2celllines exhibited increased invasion relative to LO2cell line (P<0.05). TheSMMC-7721and MHCC–97H cell lines exhibited moderate invasion (P=0.091and0.092, respectively), Compared with the LO2cell line, the elevatedTalin-1-expressing cell line, MHCC-97L, exhibited slightly reduced migration(P=0.052). The Bel-7402, SMMC-7721, HepG-2and MHCC–97H cell linesexhibited increased migration compared with LO2cell line (P<0.05). Thecolony-forming ability differed among the cancer cell lines compared with the LO2cell line (P=0.00017). The high Talin-1-expressing MHCC-97L cell line produced52colonies for a colony clone formation rate of130%(P=0.185and P>0.05,respectively). The low Talin-1-expressing BEL-7402cell line produced64coloniesfor a colony formation rate of160%, The colony-forming ability of SMMC-7721,HepG-2and MHCC–97H cell lines are between with them.CONCLUSION:1, Different types of liver cancer cells exhibit differinginvasion and migration capabilities.2, Talin-1mRNA expression differedsignificantly in different types of liver cancer cells.3, Talin-1is associated withthe invasion and migration of human liver cell lines.4, High levels of Talin-1expression are correlated with reduced invasion and migration as well as decreasedmalignancy in human liver cancer cell lines; the suppression of Talin-1promotesinvasion and migration.5, Talin1can serve as a one of predictors of primary livercancer in malignant degree.