The Relationship Between Expression Of SQLE And Prognosis In Lung Squamous Cell Carcinoma

Background and Objectiv:SQLE (squalene epoxidase) catalyzes the first epoxioxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymesas, the high expression of SQLE was closely related to a variety of tumor occurrence, development and prognosis. In this study, we analyzed the expression of SQLE mRNA and protein in patients with lung squamous cell carcinoma and benign adjacent non-cancerous lung tissues. Also we evaluated its clinicopathological and prognostic significance.Methods:The SQLE mRNA and protein expression were examined by semi-quantitative RT-PCR、fluorescence quantitative PCR and Western blot in65cases of lung squamous cell carcinoma tissues and adjacent non-cancerous lung tissues. The relationship between clinicopathologic features and prognosis was investigated.Results:SQLE mRNA and protein expression in lung squamous cell carcinoma was obviously higher than in adjacent non-cancerous lung tissues (63.07%vs44.61%, X2=4571, P=0.0348;67.69%vs38.46%,X2=11.1499, P=0.0008).The SQLE mRNA positive expression rate was not associated with gender, age, smoking, tumor size (P>0.05). SQLE mRNA expression was closely correlated with the poor differentiation, clinical stages and lymphatic metastasis (P<0.05). The SQLE was negative correlated with the overall survival rate (P<0.05)Conclusion:High expression of SQLE gene may be closely related to the occurrence and development in lung squamous cell carcinoma. SQLE expression predicts a poor prognosis and may serve as a novel lung carcinoma molecule marker.