Magnesium Sulfate On Traumatic Brain Injury In Rats And Experimental Studies Of Brain-derived Lung Injury

Objectives1、Traumatic brain injury model of rats, to explore different doses of magnesiumsulfate on neural functional recovery after craniocerebral injury in rats, cerebraledema, plasma cortisol and plasma TNF-α and brain tissue pathologicalmorphology.2、 To discuss different doses of magnesium sulfate (MgS04) on rat source sexlung injury after breathing rate, neurogenic pulmonary edema, routine bloodleukocyte count, plasma TNF-α inflammatory factors and the influence of thelung tissue pathology morphology.MethodsChoose healthy clean level in guangxi medical university laboratory animalcenter30male SD rats, weight between250-300grams, by the method ofrandom number SD male rats were randomly divided into control group,magnesium sulfate intervention group according to different dosing dose isdivided into50mg/kg group,100mg/kg and200mg/kg group, each group ofsix. Improved Feeney method is adopted to establish the model of acutecraniocerebral injury, the rat made after the success of the mould, magnesiumsulfate intervention group immediately by intraperitoneal injection of50mg/kg MgS0425%, magnesium sulfate group50mg/kg, magnesium sulfate injectiongroup100mg/kg twice injection and magnesium sulfate injection200mg/kggroup four times, getting a every8hours. Control group and model group thesame dose of saline injection and magnesium sulfate50mg/kg group injectedonce MgS04injection and magnesium sulfate100mg/kg group after2timesMgS04also injected the same dose of normal saline as control, methods200mg/kg with magnesium sulfate group.48hours after the application of improvedneurologic evaluation method for all neural function in rats and to determine thebreathing rate, after the score and respiratory frequency measurement, the heartblood, using the ACCESS immune analysis system for quantitativedetermination of rat plasma cortisol concentration, by using enzyme-linkedimmunosorbent (ELISA) detection kits TNF-α measurement, And the rat bloodroutine blood test. Determination of the brain, lung, big mouse brain contusionarea edge water content, the cortex and right pulmonary water contentdetermination of rat lung tissue. Brain tissue contusion area and left lung routineHE staining, and then observed through the rat brain tissue and the pathologicalmorphological changes of the left lung contusion area.Results1、After craniocerebral injury contusion in rats brain tissue water content,theconcentration of plasma cortisol, and concentration of TNF-α were significantlyhigher than the control group (P <0.01), nerve function score were significantlylower than the control group (P <0.05).2、200mg/kg magnesium sulfate intervention group brain water content, theconcentration of plasma cortisol, and concentration of TNF-α significantly lowerthan the model group (P <0.05), nerve function score significantly is higherthan the model group (P <0.05). 3、 In the rat brain tissue contusion area by HE staining were visible,magnesium sulfate intervention group contusion area cerebral edema degreeobviously improved compared with model group, brain contusion area, amongthem with magnesium sulfate200mg/kg group improved the most obvious.4、Model group and magnesium sulfate were breathing rate each interventiongroup was obviously higher than that of control group (P <0.01), model groupbreathing rate higher than that of magnesium sulfate200mg/kg group (P <0.05).Lung tissue water content after craniocerebral injury were higher than in controlgroup, with magnesium sulfate50mg/kg group of the most obvious (P <0.05),the difference is statistically significant.5、Every intervention group, model group and magnesium sulfate, concentrationof TNF-α,the WBC count are significantly higher than the control group (P <0.01), magnesium sulfate200mg/kg group of white blood cell count, TNF-αconcentration is lower than the model group (P <0.05).6、Model group and magnesium sulfate intervention group compared withcontrol group, terminal bronchial lumen visible inflammatory cells, was alsoseen between alveoli and alveolar inflammatory cells invasion, increase alveolus,alveolar visible and mutual confluence, thickening of alveolar walls, pulmonaryvasodilation and congestion.Conclusion1、 Craniocerebral injury early magnesium sulfate can reduce cerebral edema,reduce the concentration of plasma cortisol and TNF-α, are beneficial to therecovery of neural function, at200mg/kg magnesium sulfate group works best.2、Traumatic brain injuries can lead to brain source sex lung injury syndrome,cause breathing rate to speed up, and pulmonary edema, magnesium sulfate can reduce rats breathing rate, reduce inflammation, with200mg/kg group effect isbest, magnesium sulfate had no obvious effect on pulmonary edema.