The Distribution Of K-ras Mutation In Colorectal Carcinoma Patients In Guangxi Zhuang Minority

Objective: To analyse the distribution of K-ras gene mutations incolorectral cancer (CRC) patients in Guangxi region and the relationshipof K-ras gene mutations and clinical characteristics of colorectal cancerpatients in Guangxi region, which provide a scientific basis forprevention and treatment of colorectal cancer patients in Guangxi region.Methods: Specimens from149CRC cases who were treated bysurgery and pathology confirmed in Guangxi Medical University CancerHospital were collected from2010.1to2014.1. Mutations in residue12and13in second exon of K-ras gene in149CRC patients were detected by Amplification Refractory Mutation System (ARMS) PCR System，and the relationships between mutations and clinical characters of CRCpatients were analyzed.Results: The overall K-ras gene mutation rate of149CRC cases was19.5%(29/149). K-ras mutations among male patients was16.7%(16/96),K-ras mutations among female patients was24.5%(13/53). K-ras genemutation rate of patients (≤60years) was22.4%(22/98), K-ras genemutation rate of patients (>60years) was13.7%(7/51). In142cases ofadenocarcinoma patients, the detection rate was20.4%(29/142).19casesof K-ras mutations were observed in110cases of colorectal cancer in theHan, the detection rate was17.6%(19/108). In Zhuang Ethnic Group, thedetection rate24.1%(7/29). Mutation in the12th codon in exon2wasmore common. Gly12Asp accounts for34.5%（10/29）of all mutations.Gly13Asp mutation rate is24.1%（7/29）. Gly12Cys mutation rate is17.2%（5/29）, Gly12Val mutation rate is13.8%(4/29), Gly12Alamutation rate is6.9%(2/29) and Gly12Arg mutation rate is3.5%(1/29).Conclusions: Our data suggests that the main type mutation of K-rasdetected in CRC of patients in Guangxi is the Gly12Asp in exon, The mutation frequency is not related to sexuality, age or ethics.