The Expression Of HMGA2in Gliomas And Relative Researches

Background:Glioma is one of the most common neoplasm in central nervous system (CNS), whose morbidity behind meningioma. In consequence of the development of medical diagnose technology, such as Computed Tomography (CT) and Magnetic Resonance Imaging (MRI), glioma’s morbidity becoming more and more higher in recent years. Due to the high proliferation and invasion of glioma, the patient’s mortality rate is far away upon the other solid tumor in neurosurgical department. Because of the high invasive ability, surgeons couldn’t confirm the broundary of glioma definitely during operation. In that case, total resection of glioma is impossible. The prognosis is deadly poor with highly reccurence. In recent years, researches about biochemical diagnosis markers and gene target therapy are becoming more and more popular. High mobility group protein A2(HMGA2) is a non-histone architectural nuclear factor, whose key function is mediate the transcription of DNA. From the literatures published, we know HMGA2can accelerate tumors’ proliferation and metastasis processes. HMGA2may be a potential biomarker and rational therapeutic target for cancer.ObjectiveAnalyzing the expression of HMGA2and Matrix metalloproteinase9(MMP-9) in different grades of glioma and relationships with invasion and proliferation.Material and method57brain specimens, which including23low-grades,18middle-grades,16high-grades, were collected from neurosurgical department in provincial hospital affiliated Shandong University. The specimens were come from27men and30women who were operated cerebral resection from February2012to October2012. In addition,6specimens of normal brain tissue from intracranial hematoma resection were approached for negative control.The expression of HMGA2and MMP-9in different grades of glioma was detected by immunohistochemical staining (S-P). The mRNA expression of HMGA2and MMP-9was detected by RT-PCR.ResultsThe positive expression rate of HMGA2was glioma cell nuclear is91.2%(52/57), of which29.8%(17/57) was highly expressed (+++),47.4%(27/57)was moderately expressed (++), and22.8%(13/57) was lowly or negatively expressed (0-+). And then there was no expression in negative control samples. What’s more, the HMGA2expression degree was positive related with glioma pathological classification. The HMGA2expression was significantly positive related with expression of MMP-9(P<0.01) and Ki-67(P<0.01). The mRNA expression of HMGA2and MMP-2detected by RT-PCR was consistent with immnohistochemical staining.ConclusionThe overexpression of HMGA2may strengthen the capacity of glioma’s proliferation and invasion. HMGA2may be a potential biomarker and rational therapeutic target for glioma.