| [Objective] To investigate the expression and clinical significance of matrixmetalloproteinase-8(MMP-8) in the acute Stanford A aortic dissection.[Methods]1.Blood samples were collected from30acute Stanford A aorticdissection patients and30aortic valve replacement patients．ELISA was used tomeasure the contents of MMP-8in the plasma.2. AAD samples were taken from30AAD patients(experiment group) during aortic replacement operation and controlaortic samples were taken from30aortic valve replacement patients(control group).hematoxylin-eosin(H-E) staining was used to observe the pathological changes ofaortic wall. The tissue expressions of MMP-8in the surgically obtained samples weredetected by Western blot and immunohistochemistry.[Results]1.MMP-8in the plasma is existed in both groups, but the acute Stanford Aaortic dissection patients showed a significantly higher level of MMP-8in the plasma［（106.51±28.27）ng/ml］than the aortic valve replacement patients［（25.65±12.95）ng/ml］（P<0.05）.2. Significant lesions of aortic intima, inflammatory cell infiltration laminated edge,elastic fibers and collagen fiber fragmentation, disintegration were observed in theexperimental group, but not in the control group. In the control group, the structuralintegrity of the ascending aorta, elastic fibers and collagen fibers arranged in closeorder, no middle degeneration.3. The expression of MMP-8in the aortic tissue with experiment group is higher, butlower in the control group（P<0.05）.[Conclusion]1.The MMP-8is highly existed in the plasma of the AAD patients butlowly in the patients of control patients suggesting that it may play a role in development of the aortic disease and can be one of reference index in the diagnosisof the AAD.2. The MMP-8is highly expressed in the middle aortic lesions but lowly in the normalfibrous tissue layer suggesting that it may participate in aortic vascular remodelingand play a important role in the process of aortic dissection formation anddevelopment. |
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