Study On Relationship Among Hepatitis B Virus Markers And Content Of HBV-DNA In Feces Of Patients With Hepatitis B Virus Infection

Objective:To develop a method for HBV-DNA detection in feces, to investigate the correlationamong hepatitis B virus markers (HBVM) in serum and HBVM, HBV-DNA in feces.In order to study the significance in HBV prevalence with the spread of feces.Materials and Methods:We collected the samples offeces and serum for all patients. HBVM in serum and feceswere determined with ELISA. Using QIAmp DNA Stool kit to extract human fecalDNA. FQ-PCR were utilized for analysis the content of HBV-DNA in feces and serumwhile it were detected qualitatively by nested-PCR.Results:The positive rates of HBsAg were74%,92%in feces and serum, respectively. HBsAgwas found in feces in one patient without HBsAg in serum. In50patients, the positiverates of HBV-DNA were62%,60%in feces and serum,respectively. There was nosignificant difference between the HBV levels in feces and serum(P>0.05).Conclusion:Establish a qualitative and quantitative detection of HBV-DNA in stool approach.HBV-DNA could be detected in feces, the highest one content of2.245×108copies/ml.Feces may have contagious and can spread HBV as a medium. So it is possible thatHBV is infected via feces. It is necessary to prevent infection from blood as well asalimentary canal.