The Genetic Identify Of Hyriopsis Cumingii(Lea) And Similar Types By Random Amplified Polymorphic DNA Method And Study On The Method Of Quality Evaluation Of Hyriopsis Cumingii Software Organization

Hyriopsis cumingii sofeware organization contains rich nutrients:Polysaccharide、protein、essential amino acid and a lot of mineral inclued calcium、phosphorus、iron、manganese、vitamins and so on.Flesh of Hyriopsis cumingii is good food and medicial value,especially in Anti-tumor、immune regulation and biological activity has now become a hot topic.But by far, how to identify different kinds of software organizations, as well as how to assess the quality of their research is still in a state ofblank.The main research topics are the Genetic identify of Hyriopsis cumingii(Lea)and similar types by Random Amplified Polymorphic DNA method and study on the method of quality evaluation of Hyriopsis cumingii Software organization.Main contents include the following:1.Literature research.By referring to the literatures, we know its historic evolution andmodern research, which include origin、living conditions、characters、collection、efficacy、abstract of ancient corpus、effective recipe、components and pharmacological functions.Inaddition, the literatures about the extracting and isolating methods of polysaccharcides wereconsulted for experimental works.2.Hyriopsis cumingii and similar types of species was analysed using the randomamplified polymorphism DNA(RAPD) identification.This research directly undermolecular marker techniques similar to the genetic characteristics of the nature of DNApolymorphism Hyriopsis cumingii and similar types of species, 2 random primers weresieved from 30 random primers, which could amplify 1 to 5 fragments individually with thefragment length varying from 250 to 1000bp.RAPD can identify Hyriopsis cumingiisoftware organizations and similar types of species DNA fragments and provide methodsand techniques for identification.3.The quality evaluation system research of Hyriopsis cumingii softwareorganizations.Comparing Hyriopsis cumingii sofeware organization、waters and sediment inpond content of heavy metals, the results show that the content of heavy metals, lead andarsenic exceed the national standard, Content is 0.54mg/kg and 2.09mg/kg, butpolysaccharides by boiling water and ethanol precipitation that contain lead 0.07mg/kg,arsenic 0.24mg/kg,the results below the national standard.The results of inspecting showthat the flesh of Hyriopsis cumingii has the ability to accumulate heavymetals.However,hazardous heavy metals lead, and total arsenic, cadmium, Mercury mainly from the pond bottom sediments.Inspect the water、mineral、extract、polysaccharide and soluble protein from Hyriopsiscumingii software organizations, With polysaccharides and soluble protein contentsinspected the production、harvesting season and the impact in processing.The resultsshowed that Hyriopsis cumingii compare with other varieties has obvious exterior featuresin the appearance.Hyriopsis cumingii soft tissues containing water 82.7%, ash 1.71%, acidinsoluble 0.4 %, ash water extract 9.29 %, ethanolic extract 4.39 %, polysaccharides 4.74%, soluble proteins 1.93 %.The best desiccative technics:the tiled density is2.5 kg/m~2, thetemperature is 90℃and the desinative time is 2h, the content of polysaccharides inHyriopsis cumingii is 21.26% and proteins is 10.7%.Polysaccharides in Hyriopsiscumingii software organizations in nanjing content is higer,Hyriopsis cumingii can beharvested in 3-4 months.Hyriopsis cumingii software organizations as medicinal raw materials, providing ascientific basis for evaluating the quality of infrastructure.