Preparation Of Gadolinium Complexes Of Manganese Tetroxide Modified Nanoparticles And Its Application In Magnetic Resonance Imaging

Magnetic resonance imaging is a molecular imaging,which is widely used in themedical field. The specific contents are as follows:The first chapter is the contrast agent on the particle size of the contrast agentand synergies. Mn₃O₄nanoparticles is by pyrolysis. The particle size is by controllingthe pyrolysis temperature and time as well as use of the seed law to regulate theparticle size, achieved through a series of experiments to be successful. In particular,use of the seed law nano particle size growth from6nm to11nm, and the growthrate reach83.33%. And compare the the T1imaging results of different size Mn₃O₄nanoparticles in solution.The second chapter selected the nanoparticles of the11nm Mn₃O₄in the firstchapter, and then gadolinium chelate carboxyl to Mn₃O₄, Mn₃O₄nanoparticles withgood water solubility and stability of the gadolinium complexes modified.Then theresult of MTT method test shows that in the concentration range of the experiment,the lower toxicity of nano-particles,having a good biocompatibility.Aqueous solutionof MRI experimental results that the Mn₃O₄nanoparticles after the modification ofthe gadolinium complex with gadolinium concentration changes obtained by fittingthe r1value of24.06mM-1.s^-1,as the manganese concentration changes obtained byfitting the r1value reached3.89mM-1.s^-1,compared to DTPA-Gd commercial valueof5.45as gadolinium concentration changes obtained by fitting r1, Mn₃O₄-DTPAfitting r1With the changes in the concentration of manganeseis1.23mM-1.s^-1, thegrowth rate reached341%and216%, respectively, and the same effect is in the cellimaging,the last that an oparticles longer circulation time in vivo imaging in vivo.