Polyvinylidene Fluoride Membrane Preparation And Application Of Ethylene In The Immune Diagnosis

In this work, symmetric network poly(vinylidene fluoride)(PVDF) membranes without dense skin layer were prepared by immersion precipitation in the water/solvent/PVDF system. These PVDF membranes have the further advantage for blotting of proteins by their high protein binding capacity, thermal and chemical stability. The PVDF membranes prepared in this work could be used as solid membranes of in-vitro diagnostic reagents and Dot-blotting.The PVDF membranes were prepared by immersion precipitation of two-step coagulation baths. By using the different alcohols as the additives, the membranes of symmetric network structure were formed with a porous surface. These PVDF membranes had excellent protein binding capacity, which distributed between120μg/cm2and228μg/cm2.After the PVDF membrane was treated by hydrophilic modification, it can be used in the colloidal gold enhanced immunochromatography assay as the solid membrane. The flow velocity of the sample and the visible color on the different membranes were studied. It was found that after the membrane using ethanol as additive was treated by the buffer solution of1%STP, it could be applied to determine the HCG and DOA (drug of abuse) products, the C/T line of product appears very quikly (T line can be seen in50seconds an C line can be seen in200seconds) and the color of C/T line is strong (the T line is G8and the C line reach G9).The results indicate that the PVDF membranes prepared in this work were solid membranes to be used in the colloidal gold enhanced immunochromatography assay.After the prepared PVDF membrane using ethanol as additive was dipped into the methanol, it can be used to the Dot-blotting as the solid membrane.During the experiment,the four negative samples (5μl、10μl、15μl、20μl’ s100μg/ml mavericks serum solution) and the four positive samples (5μl、10μl、15μl、20μl、’s INF) were spotted on the membrane. The brown dot can be seen on the membrane as the positive result when the positive sample was used.And the brown dot can not appear on the membrane as the negative result when the negative sample was used.