| Chemiluminescence analysis technique showed attractive characteristics of high sensitivity, low detection limit, wide linear ranges and short analysis times. It was widely used in trace analysis. Nowadays, some new technologies such as paper-based chip, molecularly imprinting polymer, nanoparticles have been applied into the chemiluminescence analysis. In this thesis, the application of chemiluminescence paper-based chip and silver nanopartiele-initiated chemiluminescence reaction for the detection of pesticides and drugs were mainly described.This thesis is divided into two parts. The first part is the review, which introduced the development, the fabrication methods and the detection methods of paper-based chip. Then the principle, the synthesis method and application of molecularly imprinted polymer were described in the review.The second part is as follows:1、A molecularly imprinted immobilized paper-based chip for chemiluminescence detection of organophosphate pesticidesPaper-based microfluidic devices are an alternative technology for fabricating simple, low-cost, portable and disposable platforms for clinical diagnosis and environmental monitoring. In this paper, Molecularly Imprinted Polymer (MIP) of dichlorvos (DDV) was synthesised on the paper surface by using DDV as the template molecule, Methyl Acrylic Acid (MAA) as functional molecule and ethylene glycol dimethacrylate (EGDMA) as cross-linking agent. For the paper was clapped inside two glass slides, the thickness between two glass slides can be controlled precisely, the quantity of DDV MIPs which was synthesized on paper surface can be precisely achieved. A paper-based MIP microfluidic chip was designed for the determination of residual dichlorvos. Under the optimal conditions, the liner range of DDV is1.0×10-9～1.0×10-7g/mL with a detection limit of6.0×10-10g/mL and the recovery was between90.0%-108.0%. The method has been successfully applied for the determination of the dichlorvos in vegetables and fruits.2、 Determination of fursultiamine based on silver nanoparticle-catalyzed luminol chemiluminescence system. A highly sensitive chemiluminescence (CL) method for the determination of fursultiamine was developed based on the enhancement of CL intensity of luminol-H2O2-fursultiamine system by silver nanoparticles (AgNPs). The luminophor in this system was excited state3-aminophthalate anion and the maximal emission of the CL spectrum was at425nm. Under the selected experimental conditions, a linear relationship was obtained between the CL intensity and the concentration of fursultiamine over the range from1.0×10-8to1.0×10-5g/mL with a detection limit of4×10-9g/mL. The relative standard deviation (RSD) for the determination of fursultiamine (1.0×10-6g/mL) was1.9%(n=9). This method has been successfully applied to the determination of fursultiamine in pharmaceutical samples.3、Determination of nitrofurans in feeds based on silver nanoparticle-catalyzed chemiluminescence.A highly sensitive chemiluminescence (CL) method for the determination of nitrofurans (NFs) was developed based on the enhancement of CL intensity of luminol-H2O2-NFs system by silver nanoparticles (AgNPs). It was supposed that the oxygen-related radicals of OH·and O2·-could be produced while NFs reacting with H2O2. Furthermore, the enchancement mechanism was originated from the reinforcer of AgNPs which could catalyse the generation of the OH·radical. Then OH·radicals react with luminol anion and HO2-to form luminol radical (L·-) and superoxide radical (O2·-). The excited state3-aminophthalate anion was obtained in the reaction of L·-and O2·-, which was the emitter (luminophor) in the luminol-H2O2CL reaction system and the maximal emission of the CL spectrum was at425nm. The experiments of scavenging oxygen-related radicals were done to detect these reactive oxygen species participated in the CL reaction. The limits of detection (LOD)(S/N=3) were8×10-7g/mL for furacilin,8×10-8g/mL for furantoin,4×10-8g/mL for furazolidone and2x10-7g/mL for furaltadone respectively. The proposed method was successfully applied to the determination of NFs in feeds and pharmaceutical samples. |
PDF Full Text Request