With the decrease of unrenewable resources and step-by-step depravation of environmental quality, the development of renewable biomass energy attracts extensive concerns. Biomass resource, as a renewable and sustainable resource, which attracts more and more attention because of extensive distribution in nature and good ecological benefits. It is expected to be a fine substitute for liquid fossil fuel and chemical intermediates. In recent years, more and more researchers are keen to identify and study chemical or biological transformations to convert biomass into biofuels and feedstock chemicals. In contrast to other renewable energy resources (solar, thermal, tidal, wind, hydro, etc.), biomass is the only renewable energy resources of fixed carbon, which is essential for the production of liquid hydrocarbon fuels and chemicals. Therefore, developing and utilizing renewable energy is a development tendency of energy market in our country.Methyl levulinate has extensive uses in the chemical industry, which is usually synthesized through esterification of levulinic acid, direct alcoholysis of biomass, alcoholysis of furfurol. Methyl levulinate is a class of short chain fatty ester with numerous potential applications either in the flavoring and fragrance industry or as additives for diesel and biodiesel transportation fuels. So methyl levulinate is considered as a new energy chemical.In this thesis we studied alcoholysis reaction of fructose to methyl levulinate in methanol solvent, and investigated catalytic performance of homogeneous two type acid catalysts i.e. copper trifluoromethanesulfonate and phosphotungstate in fructose alcoholysis reaction. The main results are as follows:1. The catalysts with different iron content phosphotungstate were prepared by double decomposition reaction and the catalysts were characterized by XRD and FTIR. The catalytic activities of the phosphotungstate with different iron content in the alcoholysis reaction of fructose to methyl levulinate were studied. The experiment results show that the catalytic activity of Fe-HPW-1catalyst was significantly higher than other catalysts. The influences of various reaction conditions (such as reaction temperature, reaction pressure, reaction time, catalyst amount, reaction substrate) on the alcoholysis of fructose to methyl levulinate have been investigated. It was found that under the reaction condition of a nitrogen pressure2MPa, amount of fructose0.75g and catalyst0.48g, reaction temperature of130℃for2h, the fructose was converted completely, and a maximum yield methyl levulinate of73.7%was obtained over the Fe-HPW-1catalyst.In addition, the Fe-HPW-1catalyst is stable and can be reused up to a minimum of five cycles.2. The copper trifluoromethanesulfonate was also used as Lewis acid catalyst to catalyze alcoholysis of fructose to methyl levulinate. The influences of various reaction factors including reaction pressure, reaction temperature, reaction time, catalyst dosage on the yield of methyl levulinate were investigated. At an amount of fructose0.75g and catalyst0.36g, a nitrogen pressure of2MPa, a reaction temperature of120℃and with24g methanol as a reactant for2h, a conversion of fructose100%with88.1%yield toward methyl levulinate were obtained on copper trifluoromethanesulfonate catalyst.