Genetic Structure Of Chinese Coastal Portunus Population

The swimming crab Portunus trituberculatus, which has high economic value, is mainly distributed in the warm sea areas of China, Japan and Korea et al., and in China, even throughout every sea areas. The subfamily Portuninae is the largest and the most widely distributed subfamily in the family Portunidae. They are commonly found near the shore of China, and many species are exploited commercially, which are the famous large-scale edible crabs. We discussed the heredity structure difference among the Portunus trituberculatus populations of different sea areas, and the diversity and phylogenetic relationship of Portuninae.We got13Portunus trituberculatus specimen from different locations, and almost1241bp of them in the D-loop sequence of mtDNA. The average genetic distance is0.025, and the phylogenetic relationship which was reconstructed by methods of NJ, ME and MP showed that there was obvious gene exchange among the population of Chinese coast, and the population from different sea areas combined. For the breeding, cultivation and resources conservation, it’s urgent to analyze Portunus trituberculatus’s seeding gene bank, reveal its heredity background, in order to confirm reasonable multiplication policy, set down scientific protective measures, guarantee this eminent seeding’s sustainable use and healthy development according to the population’s heredity structure.Furthermore, we got10swimming crabs’ specimen and almost318bp,538bp and676bp of them in12S rRNA,16S rRNA and COI sequences of mtDNA respectively. The average genetic distance (including the data from GenBank) is0.128,0.128and0.164, and the phylogenetic relationship can be determined by observing the morphology of the Portuninae crabs. Comparing of12S rRNA,16S rRNA and COI sequences, the result showed that it had high bootstrap value and the trees were similar to each other. The result of molecular level was approximately same with the classification result based on morphological analysis, and so we could determine the phylogenetic relationship of the swimming crab subfamily Portuninae basically.Our research showed that mtDNA D-loop gene was feasible to use as a molecular marker among the Portunus trituberculatus populations’seeding identify and12S rRNA,16S rRNA and COI gene sequences were doable to inference the phylogenetic relationship of Portuninae. Our molecular evolution study, not only has significance in crab’s evolution theory, but also has instruction function in the swimming crab’s exploiture and seeding resources conservation.