Vice Pig Identification And Vaccination Haemophilus Species With Characteristics Of Separation

Haemophilus parasuis is parasitic on the porcine respiratory tract pathogens causing pig Lazeshi disease, in order to cause swine polyserositis, arthritis and meningitis as characteristic; the disease exists widely in the world, in our country with the industry development of the disease has brought the huge economic loss; this study through the pathogen of Sichuan area of Haemophilus parasuis, biological characteristics, vaccine strains for research, laid the foundation for diagnosis, Sichuan area of Haemophilus parasuis disease prevention and controlThis research is mainly divided into the following three parts:1Isolation and identification of Haemophilus parasuisThis study from the Sichuan area large-scale pig farms for the suspected of Haemophilus parasuis disease were isolated4strains of bacteria, these strains by colony morphology, staining properties, biochemical reaction, PCR identification and sequencing identified as Haemophilus parasuis.2Haemophilus parasuis growth characteristics ofLine SC-1to get this room isolation and identification of Haemophilus parasuis isolate Sichuan as the representative of biology of the bacterium. The results show that the Haemophilus parasuis respectively in TSA solid medium and TSB liquid medium was the best; the liquid TSB medium growth curve showed live bacteria Haemophilus parasuis in16-20h number reached its peak, so for the preparation of Haemophilus parasuis out the best harvest time live vaccine bacteria liquid is appropriate at about18h.3Haemophilus parasuis isolates screened forThe acute toxicity to mice as animal model, by way of intraperitoneal inoculation, the median lethal dose of Sichuan area of Haemophilus parasuis strains isolated in the laboratory (LD50) determination, results showed that half of the SC-1, SC-2, SC-3, SC-4strains respectively2.20×109CFU、2.07×109CFU、2.01×109CFU、2.16×109CFU, according to the above test results of screening of virulence virulent SC-3as a kind of strains; SC-3preparation of inactivated vaccine based on the target animal (pig) in accordance with the observation of the temperature and the state of mind of normal dose and dose after inoculation, results show that the preparation of SC-3inactivation is safe; the Haemophilus parasuis SC-3strain preparation of inactivated vaccine at28days of age10piglets, three weeks plus free time, plus free after14daily SC-3strain after intraperitoneal injection of virus attack, results from the SC-3strain preparation unit inactivated vaccine protection rate for9/10.