Correlation Between Wild And Cultivated SD Odor And Color Quality

Objective:1. Using colorimeter and electronic nose technology to create wild and cultivated Fangfeng samples digitized color and odor, objective analysis.2. Analysis odor and color quality of Fangfeng correlation.Research:1. Sample collectionCollect samples of47batches Fangfeng, wild Fangfeng which samples17batches,30batches cultivation Fangfeng samples. Wild Fangfeng mainly from Taikang Heilongjiang, Daqing and Inner Mongolia Hailar, Tongliao, Manchuria and other places. Cultivated Fangfeng mainly from Hebeianguo, Inner Mongolia and Heilongjiang some areas.2. Character identificationAccording to the2010edition of "Chinese Pharmacopoeia" experiments on Fangfeng samples collected were identified, determine the sample collection is the dried root Umbelliferae Saposhnikovia divaricata (Turcz.) Schisck. Fangfeng in the wild and cultivated traits quite different, mainly in herbs shape, color, texture, broken face, smell and so on.3. Microscopic identificationFangfeng by observing the wild and cultivated cross sections, longitudinal sections and found cork layer thickness, the number of tubing color, xylem conduit arrangement are also different.4. High Performance Liquid Chromatography Determination of active ingredients in the FangfengBased on the2010edition of "Chinese Pharmacopoeia","determination" in the Ci-micifuga glycosides and4’-O-beta-Glucopyranosyl-5-O-Methylvisamminol were determin-ed. Cultivated Fangfeng Cimicifuga glycosides average content of0.169%,4’-O-beta-Glucopyranosyl-5-O-Methylvisamminol content of0.165percent on average, Cimicifuga glycosides and4’-O-beta-Glucopyranosyl-5-O-Methylvisamminol content of O.334percent. Wild Fangfeng Cimicifuga glycosides average content of0.293%,4’-O-beta-Glucopyranosyl-5-O-Methylvisamminol content of0.159percent on average, Cimicifuga glycosides and4’-O-beta-Glucopyranosyl-5-O-Methylvisamminol average alcohol content of0.452%. Overall, the activeingredient is higher than the cultivation of cultivated Fangfeng, storm.Inner Mongolia Fangfeng,Cimicifuga glycosides and4’-O-beta-Glucopyranosyl-5-O-Methylvisamminol average content was0.404percent. Heilongjiang Fangfeng Cimicifuga glycosides and4’-O-beta-Glucopyranosyl-5-O-Methylvisamrninolcontent of0.414percent average,the average content of Hebeianguo cultivationFangfeng Cimicifuga glycosides and4’-O-beta-Glucopyranosyl-5-O-Methylvisamminol was0.327%.Description Heilongjiang Province Fangfengproof best quality, followed by Inner Mongolia Fangfeng, Hebeianguo Fangfeng.5. Establish HPLC fingerprint of wild and cultivated Fangfeng in the overall analysis ofthe compositionA preliminary study of the chemical composition of wild and cultivated Fangfeng fingerprints, wild Fangfeng and established a total of six peaks, wild Fangfeng and the similarity between0.777-0.953, a comprehensive analysis of15wild Fangfeng liquid chromatogram, retention time RSD was0.09-0.18%, peaks area RSD was0.407-0.612%.Cultivated Fangfeng established a total of10peaks, cultivation Fangfeng similarity between0.784-0.995. Comprehensive analysis of15cultivars Fangfeng liquid chromatogram, retention time RSD value of0.03-0.68%, peaks area RSD was0.298-0.665%.6. GC-MS analysis of volatile components for the FangfengIn accordance with the2010edition of "Chinese Pharmacopoeia" an appendix XD method to calculate six different habitats of wild and volatile oil yield, yield cultivation of wild Fangfeng storm samples were0.3mL/100g,0.38mL/100g,0.42mL/100g. Fangfengbreak cultivated volatile oil yields were0.1mL/100g,0.1mL/100g,0.2mL/100g. Fangfeng volatile oil were identified49compounds, mainly by chain aliphatic compounds, aromatic compounds and terpenoids, with the highest content of aliphatic compounds. Fangfeng in the volatile oil from different sources contain Panaxynol,4-hydroxy-4-methyl-2-pentanone, octanal,(S)-1-methyl-4-(5-methyl-1-methylene-4-hexenyl)cyclohexene,(R)-1-methy1-4-(1,2,2-trimethyl-cyclopentene) toluene,[9,12]-ten eight carbon diene methyl six compounds, but different sources and origin of the Fangfeng volatile oil components are different.7. Colorimeter color samples for determination of Fangfeng and AnalysisColorimeter using35batches of the collected samples Fangfeng, where wild specimen13batches,22batches cultivated sample color measurement, using SPSS software to color values L*, a*, b*, E*ab value of the variable, cluster analysis on Fangfeng samples. Apparent from the clustering tree to L*, a*, b*, E*ab value indicators will be divided into two types of samples. On the part of a wild Fangfeng, the lower part of a cultivated Fangfeng. Wild Fangfeng of E*ab values in the range59.69-65.33, E*ab values cultivated in the63.88-78.32Fangfeng. Cropping patterns color values clustering results are consistent with the Fangfeng samples and Fangfeng associated with powder color samples.8. Electronic nose technology for Fangfeng measurement and analysis of samples odorUsing electronic nose technology to build on the growth of Fangfeng in different ways DFA model, wild and cultivated by the DFA model can be Fangfengproof, proven (>90%) samples of38groups, accounting for95%; unauthenticated have two sets of samples,5%, most of the data set gives the correct identification, description DFA model has a certain significance in determining the application of Fangfeng in different growth patterns. Similarly, DFA model is also applicable to the origin of Inner Mongolia and Heilongjiang two wild and cultivated goods products.DFA model is different origin discrimination Fangfeng shows:Tongliao Fangfeng, Hailar Fangfeng with Heilongjiang Fangfeng difference. Inner Fangfeng samples collected from the market, businesses are unsure specific origin, and therefore specific information such samples is unknown. Cross area of Heilongjiang and Inner Mongolia Fangfeng amples samples, may be due to some samples on location in Inner Mongolia Fangfeng and Fangfeng in Heilongjiang short distance, so the failure to distinguish between the two Fangfeng samples.Establish SIMCA model validation samples are Fangfeng and cultivated wild Fangfeng samples specified target group agreed. All herbs Fangfeng identify an acceptable standard within the region, for the most part in the cultivation of Fangfeng samples to identify acceptable within the region, cultivated Fangfeng sample is not recognized as FFB-3(Inner Mongolia), FFB-11(Hebeianguo), FFB-15(Heilongjiang), these samples were from different origin, not by identifying wild Fangfeng samples, indicating that the model samples cultivated and wild Fangfeng storm created by the existence of differences.Innovations:1. For the first time using a colorimeter on the organizational structure of wild and cultivated Fangfeng outer layer of color formation was measured to establish wild and cultivated products total value of E*ab color range.2. For the first time using electronic nose technology to build wind DFA models with different growth patterns of the sample, the models distinguish wild and cultivated Fangfeng samples.3. Analysis samples of wild and cultivated Fangfeng "color","gas" with the active ingredient correlation.