Using PCR-DGGE Method For Determining The Adjustment Effect Of LBP On Mouse Intestinal Flora

Objective： PCR-DGGE separation of intestinal bacteria using molecularbiology16SRNA V3variable region PCR products by DGGE profile strips withanalysis, combined with traditional microbiological isolation, culture,identification methods, of of wolfberry polysaccharides on intestinaldysbiosis modelrole mice adjusting.Methods： First lincomycin hydrochloride in mice, making the mouseintestinal dysbiosis model. Subsequent model mice were divided into LiveBifidobacterium Preparation group, natural recovery group and LBP group,were given the appropriate medication. All mice were sacrificed seven daysafter gavage, ileocecal stool specimens collected. Serial dilutions ofstool specimens, and then traditional live bacteria culture and DNA wasextracted and PCR-DGGE method for detection of mouse intestinal flora DNA.Finally, all data collection, analysis, observation of the effect of ofwolfberry polysaccharides on the treatment of intestinal dysbiosis mice.Results: Traditional live bacteria culture results show: the mouseintestinal bifidobacteria, lactobacilli increases, cloacae,Enterococcus decline the wolfberry polysaccharide group and LiveBifidobacterium Preparation group and natural recovery group, thedifference was very significant (P <0.01). The wolfberry polysaccharidegroup compared with Lizhuchangle group, the difference was significant(P <0.05). PCR-DGGE results showed that: a stool sample total DNAconcentration measured value, the the wolfberry polysaccharide group andLive Bifidobacterium Preparation group than natural recovery group (P<0.05), DGGE diversity index analysis, the Live BifidobacteriumPreparation group and the wolfberry polysaccharide group is greater than the natural recovery group and normal control group, the difference wasstatistically significant (P <0.05); the wolfberry polysaccharide groupthe slightly superior Live Bifidobacterium Preparation group, but therewas no significant difference.Conclusions：Mice by oral hydrochloric Lin lincomycin can be successfullyproduced model mouse intestinal dysbiosis. Traditional live bacteriacultures and PCR-DGGE results prove, LBP has a significant role in theadjustment of intestinal dysbiosis mice. PCR-DGGE technique has acomprehensive, high sensitivity, time-saving, no microbial culture, hassignificant advantages over the traditional culture method. PCR-DGGEtechnique diagnosis of dysbiosis the evaluation microecologicalregulator role a powerful tool.