Ruthenium (Ⅱ) Complexes Synthesis, And DNA And Its Activity Against Tumor Research

Ruthenium(II) polypyridyl complexes play a key role in the fields of photophysics,photochemistry, electrochemistry and molecular assembling, etc. In particular, aconsiderable attention has been attracted in their extensive potentials as DNA structurerecognition, DNA mediated electron transfer and anticancer drugs, and so on. Therefore,tremendous interest has been aroused to the study of Ruthenium(II) polypyridinecomplexes during the past decades. This dissertation concretely consists of the followingfour chapters:In chapter1: The theoretical foundation, the research methods and prospect ofinteraction between Ruthenium(II) polypyridine complexes and DNA were summarizedIn chapter2: A novel ligand DNPIP(2-(2,4-binitro)imidazo[4,5-f][1,10] phenanthroline)and its complexes have been synthesized and characterized. The interaction of thesecomplexes with calf thymus DNA was investigated by spectroscopic, viscositymeasurement and thermal denaturation. The results show that these complexes bind to DNAvia an intercalative mode. At the same time, the nature of reactive intermediates involved inthe efficient DNA phototcleavage is likely to involve a mechanism based on1O2,In order toconfirm this result, the experiment for detecting the formation of singlet oxygenphoto-sensitized by complexes was carriedout. To further validate themeaningful bindingstoichiometries obtained betweencomplexesand DNA,continuous variation analysis (Jobplot) was investigated. The DNA condensation capacity of these complexes was determinedby gel retardation experiments.The research of MTT, apoptosis assay, cell uptake and flowcytometry show these complexes has a certain antitumor activityIn chapter3: The chlorineatoms have been introduced into the intercalative dppzligand and five novel Ru(II) complexes have been synthesized and characterized.Thestudies of spectroscopic, viscosity measurement and photoactivated cleavage suggest thesecomplexes interact with DNA through intercalative mode.The experiment of gel retardation experiments show the five complexes has differently condensed DNA capacity. Theresearch of MTT, apoptosis assay, cell uptake and flow cytometry show these complexes ofdcdppz has strong antitumor activityIn chapter4: A novel ligand NHPIP(2-(4-hydroxyphenyl-3-nitryl) imidazo [4,5-f][1,10] phenanthroline) and its complexes have been synthesized and characterized. Theinteraction of these complexes with CT-DNA was investigated by spectroscopic andviscosity measurement, the results indicatethat the three complexes bind to DNA via anintercalative mode. The antitumor activity of these complexes were studied by MTT andapoptosis assay. Ahydroxyl radical scavenging activity of these complexes wereinvestigated.