| The study of exercise-induced fatigue is the hot topics of Sports Medicine and Sports Science, so it is particularly important to find safe and effective anti-fatigue drugs. With the unique advantages of safety, low adverse effect and almost no prohibited products for human body, Traditional Chinese Medicine used as anti-fatigue drugs has been aroused wide concern, more and more researches are trying to find natural anti-fatigue drugs from Traditional Chinese Medicine.Bushenyishou pian as compound Traditional Chinese Medicine is composed of Epimedium Herb, Barbary wolfberry fruit, Glossy ganoderma, and Rhizoma polygonati and so on. The ingredients such as Epimedium Herb, Barbary wolfberry fruit, Glossy ganoderma have been shown to have significant anti-fatigue effect. The anti-fatigue effects of Red ginseng, Liquorice root and Rhizoma polygonati also have been reported. The object of this article is to investigate the anti-fatigue effect of Bushenyishou pian on exhaustive swimming exercise mice. The contents of experiment include two parts. The first part is aim to discuss the anti-fatigue effect of different doses of Bushenyishou pian (1,3,9g/kg). Serum biochemical parameters including ALT(alanine aminotransferase), AST(aspartate transaminase), MDA (malondialdehyde), BUN (blood urea nitrogen), SG (serum glucose) were measured, and PAGE (polyacrylamide gel electrophoresis) of LDH isozyme in blood was analysed. The suitable dose of Bushenyishou pian determined from the first experiment was used in the second part. ALT, AST, MDA, BUN and SG in blood serum, SOD (superoxide dismutase) and MDA in brain, SOD and MDA in heart, SOD, MDA and glycogen in muscle, SOD, MDA and glycogen in liver were measured. And electrophoresis of LDH isozyme in serum, muscle, heart, brain and liver was analysed to explore some mechanism on anti-fatigue effect of Bushenyishou pian in mice.The results of the first experiment show:The middle dose of Bushenyishou pian (3g/kg) can obviously prolong the exhaustive swimming time of mice, significantly decrease the activities of ALT and AST and the concentrations of MDA, BUN and the total activity of LDH isozyme compared with that in control group. But the concent of SG has no significant difference with the control group. All of the results demonstrate that Bushenyishou pian has anti-fatigue effect.The results of the second experiment show:1. Bushenyishou pian can significantly prolong the swimming time, the extension rate is27.76%. The result shows Bushenyishou pian can increase exercise endurance of exhaustive swimming mice.2. Bushenyishou pian can significantly decrease the activities of ALT and AST, the levels of MDA and BUN in blood serum. The results reveal Bushenyishou pian can accelerate the recovery of body in the state of exhaustion and recovery.3. Bushenyishou pian can significantly improve the activity of SOD enzyme in liver, heart, muscle and brain and decrease the levels of MDA in the state of exhaustion and recovery.4. Bushenyishou pian can inhibit the excess reducing of SG, glycogen in liver and muscle at exhaustion, and accelerate the restoring of glycogen in liver and muscle.5. From the electrophoretogram analysis of serum and organs, we can know Bushenyishou pian can change the distribution and concent of LDH isozyme to the direction that can protect the body and promote the athletic ability.In conclusion, Bushenyishou pian can improve the athletic ability of mice and promote mice to recover from fatigue rapidly by alleviating the oxidative damage of organs and regulating the body’s metabolic state. Whether Bushenyishou pian has anti-fatigue effect on the human body and improve people’s atheletic ability is worthy to be studied further. |
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