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Manipulation Of Rabbit Lumbar Disc Herniation Model β- Endorphin And Serum Prostaglandin E 2 Of

Posted on:2014-05-26Degree:MasterType:Thesis
Country:ChinaCandidate:X J DongFull Text:PDF
GTID:2264330425474711Subject:Acupuncture and Massage
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Purpose:Massage the nerve roots in the lumbar intervertebral discprotrusion inflammatory lesion model in rabbit serum β-EP and PGE2content and by HE dyeing observation of the nerve root,the nerve root todiscuss massage lumbar intervertebral disc protrusion therapeutic effectand mechanism of inflammatory injury.Material and method:O-level rabbit24, male and female half and half.All the animals were randomly divided into3groups, each group ofeight.Methods the treatment group, model group,blank control group.Thebuilding method of the experiment using jiang zhuang building methods,copy the rabbit model of lumbar disc prolapse.Within one week after theoperation take out stitches after basic to heal the wound.At the same time start to massage therapy treatment group.Choose point,press, kneading, treatment part selects the waist clip ridge cave andbilateral acupuncture point.With medium strength of the stimulus quantity,clip on both sides of the spine ridge cave "tendons knot","reinforcedpoly "(pathological phenomena such as area and bilateral acupuncturepoint do technique, each rabbit each treatment8~10min (according toknead ridge point5-8minutes, click on the bilateral acupuncture point2-3minutes), once a day.1,7,14,28days after paralysis of lower limbswas detected in recovery, the30th day after blood to be put to death allthe rabbit model.By using enzyme-linked immunosorbent (ELISA) detectionmodel of rabbit serum β-EP and PGE2for testing.And as compared with themodel group and blank group.The experimental results adopt SPSS10.0software package for statistical analysis.Measurement data to mean+/-standard deviation (+/-S), according to multiple sets of meandifferences compared with single factor analysis of variance. Results:Compared with control group, model group rats serum β-EP andPGE2levels increased significantly (p <0.01), there is statisticalsignificance;Technique compared with model group, the treatment grouprats serum β-EP, a significant rise in content, PGE2levels decreasedsignificantly (p <0.01), with statistical significance.Conclusion:①massage the serum β-EP on lumbar intervertebral discprotrusion has higher effect②Massage the PGE2in serum for lumbarintervertebral disc protrusion has lower effect.③Massage for pain causedby lumbar disc prolapse has better therapeutic effect.
Keywords/Search Tags:The massage, Lumbar disc disease, β-EP, PGE2
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