EML Study Chemical Constituents Of The High-speed Countercurrent Chromatography

Morus alba L leaves, as an edible and medicinal plants, is widely used in the treatment and prevention of diseases in chinese and foreign folk. Numerious studies show that mulberry leaves contain a variety of functional ingredients, such as flavonoids, alkaloids, phenylpropanoids, terpenoids, phytosterols, sugars and volatile oils, ect.. Meanwhile, they also have hypoglycemic, lipid-lowering, antioxidant, protecting the cardiovascular system, anti-bacterial, anti-tumor and other pharmacological effects. Currently, the chemical constituents of mulberry leaves are mainly isolated by using conventional separation methods, such as resins, gel, ect., which present seriously dead adsorption, low recovery, and other problems. Thus, it has important significance to establish a rapid and efficient method for the separation and purification. In this study, HSCCC combined with HPLC was used to isolate the ethyl acetate extract of mulberry leaves, and the compounds of coumarin, terpenoids, Phenyl-benzofuran were obtained.This paper is composed of three parts:1. OverviewAn overview of the study on chemical constituents and pharmacological effects of mulberry leaves. Studies on chemical constituents show that mulberry leaves mainly contain flavonoids, alkaloids, phenylpropanoids, terpenoids, phytosterols, sugars and volatile oils and other functional ingredients. Pharmacological studies show that mulberry leaves have a variety of pharmacological effects, such as hypoglycemic, anti-oxidation, lipid-lowering, anti-tumor, anti-bacterial, anti-viral, myocardial protection systems, ect.. Currently, conventional separation methods, such as resins, gel, HPLC, ect., are used to isolate the chemical constituents of mulberry leaves.2. Extraction and separation of mulberry leaves’chemical componentsHSCCC was used to isolated the ethyl acetate extract of mulberry leaves by using petroleum ether-ethyl acetate-methanol-water (6:10:7:9) for the two-phase solvent system, get1-7fractions;HSCCC was used to isolated the fractions by using petroleum ether-ethyl acetate-methanol-water with different proportion for the two-phase solvent systemand, isolated (6S,9R)-6,9-dihydroxy-3-oxo-a-violet alcohol, icarisideB1aglycone and LL-2-2from fraction2, isolated7-hydroxycoumarinand Moracin M from fraction4, isolated Moracin P from fraction5, isolated LL-6from fraction6, isolated LL-7from fraction7,The fraction3was separated by HPLC to obtain scopoletine and LL-3-2;The failed eluted samples were continuously separated by HPLC to obtain palmitic acid, and LLG-1and LLG-6, which are still in spectral analysis.3. SummaryThis study established a new technique of HSCCC-HPLC for isolation and purification the chemical constituent from Morus alba L leaves,12compounds have been isolated,7compounds were identified, such as (6S,9R)-6,9-dihydroxy-3-oxo-α-violet alcohol, icarisideB1aglycone, Scopoletin,7-hydroxycoumarin, Moracin P, Moracin M, ect., were obtained. HSCCC-HPLC is a very powerful technique for the isolation and purification of chemical constituent from Morus alba L leaves, This work makes a foundation for further studies on the pharma cological research and quality control of Morus alba L leaves.