Study On Qingkailing Injection Monosaccharides And Disaccharides Compounds

As a kind of national protected traditional medicine, Qingkailing injection is with the effects of clearing away heat, expelling superficial, eliminating phlegm, dredging collaterals and inducing resuscitation. The formula of Qingkailing injection is composed of cholic acid, hyodeoxycholic acid, Concha Margaritifera, Radix Isatidis, Cornu Bubali, baicalin, Fructus Gardeniae, and Flos Lonicerae Japonicae. In which, baicalin, hyodeoxycholic acid and cholic acid are extracts, while Radix Isatidis, Fructus Gardeniae and Flos Lonicerae Japonicae are herbs. As the common compounds in herbs, saccharides include polysaccharides, oligosaccharides, disaccharides and monosaccharides. The literatures show that, the research of monosaccharides and disaccharides of some injections has been reported, but they have not been reported in Qingkailing injection. Therefore, the research of saccharides will have a certain value in the quality control of Qingkailing injection.Monosaccharides and disaccharides, in Qingkailing injection were studied systematically in this project. An HPLC-ESI-MS" method for analyzing monosaccharides and disaccharides in Qingkailing injection was developed, and the method for determination had been established by using HPLC-ELSD technology. What’s more, we had explored the presence of such compounds in different manufacturers and different batches of Qingkailing injection. In addition, the presence of these compounds of Kudiezi injection also had been studied. The following is the main research contents and results:1. The analysis and identification of monosaccharides and disaccharides in Qingkailing injectionAt this part, the technology which used to analyze and identify monosaccharides and disaccharides was developed by HPLC-ESI-MSn. Retention time of each component was obtained, together with the molecular weight and MS/MS fragmentation information for using the developed method. Ultimately,1disaccharide and3monosaccharides were identified in the Qingkailing injection.2. Determination of monosaccharides and disaccharides in Qingkailing injection by HPLC-ELSDAn HPLC-ELSD method for determination of monosaccharides and disaccharides of Qingkailing injection was developed, the Qingkailing injection of16different batches and eight different manufacturers were determined in this part, the presence of monosaccharides and disaccharides in Qingkailing injection were preliminarily evaluated. Thus it can provide theoretical basis for its quality control.3. The analysis of monosaccharides and disaccharides in Kudiezi injection Reference the research methods of saccharides in Qingkailing injection, few studies of monosaccharides and disaccharides of Kudiezi injection were conducted. The results show that,1disaccharide and2monosaccharides were identified in Kudiezi injection. For this chapter, an HPLC-ELSD method for determination of monosaccharides and disaccharides of Kudiezi injection was developed, the content of fructose, glucose and sucrose of9different batches was determined.