| The Four Characteristics of Herbs, which composed of cold, heat, warm and cool, is the most important theory in traditional Chinese medicine and the guidance in clinical prescription. However, there are still some questions cause attentions of doctors in all dynasties. Such us:if the Four Characteristics of Herbs objectively existence? Is there an objective and effective differentiate criteria and method? What are the biological activity and material basis of the Four Characteristics of Herbs? This paper was to investigate the Objective quantitative index of traditional Chinese medicine attribute based on Chinese herb pharmacological theory and reference, by the experimental research of growth and metabolism and absorption and release heat of Coptis chinensis, radix paeoniae alba, rhizoma zingiberis and Platycodon grandiflorum. The main content and conclusions are as follows:(1). The three reviews have summarized literatures related to traditional Chinese medicine fever attributes, the current situation and application of race amount of heat and textual research of literatures on four classical herbs in typhoid essay. After textual research of previous dynasties medica ancient books of fever attributes and clinical applications and modern research of the four herbs mentioned above, we have found that herbs belong to the cold nature or cool nature were effective on ameliorating of heat syndromes and herbs belong to the heat nature or warm nature were effective on ameliorating of cold syndromes. Herbs of cold nature had a inhibitory action of the metabolic system while the herbs of heat nature had a auxo-action on the metabolic system. We could produce evidence to quantizate the drug properties of Chinese herbs though the quantization and visualization of thermal energy by direct monitoring of the thermodynamic process based on the microcalorimetry.(2)We established a reasonable experimental system and procedure in a regulatory standardized and repeatable principle:5×105cfu/mL E.coli seeded in4925μl LB culture medium into a20ml ampere glass bottle, supplemented with water decoction of Chinese herbs filtered with80aperture nylon mesh in a concentration of0.01、0.02、0.03g/mL. Statistical analysis was assessed by repeatable experiments in the same method. The results indicated that the heat release effects of bacteria were inhibited by the rhizoma coptidis and radices paeoniae alba in a dose-dependent manner but improved by the rhizoma zingiberis, the difference is statistically significant。 The power of the first wave peak value of Platycodon grandiflorum group was significantly higher than the control group. Compared with the Platycodon grandiflorum group, the power of the first wave peak value was significantly increased in the rhizoma zingiberis group. In contrast, The power of the first wave peak value of radices paeoniae alba group was significantly lower than the control group but higher than the rhizoma coptidis group. The effects on the bacteria were stronger in rhizoma zingiberis and rhizoma coptidis groups than the Platycodon grandiflorum radices paeoniae albagroups. The total output of heat of rhizoma zingiberis and Platycodon grandiflorum showed a increased trend compared with the radices paeoniae alba group; meanwhile compared with the rhizoma coptidis and radices paeoniae alba groups,a higher trend towards the period of output of heat in the rhizoma zingiberis and Platycodon grandiflorum groups.We corroborated the Growth of exothermic process of the E.coli was impacted with the four herbs from Growth rate, the inhibition rate and power of the first wave peak value. Moreover we found a different trend of total time and total output of heat between the cold nature herbs and heat nature herbs. Meanwhile we clarified that the impact on the heat production of E.coli was different between the doses of herbs. However it needs to be further investigated that whether the impact on bacteria is the expression of the property of herbs. |
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