Based On The Establishment Of Quantitative PCR Technology To Detect Genetic Polymorphisms Associated Antineoplastic Methods

With the rapid development of pharmacogenomics recently, personalized medicine is gaining increasing attention in the filed of cancer treatment. Owing to the differences existed in the drug metabolism, the response to drugs and their therapeutic effect varied among individuals. According to researches, these variation are closely associated with the genetic polymorphisms of individuals. By realizing personalized medicine, every patient can be prescribed most properly and in the long run the therapeutic effect can be improved meanwhile reducing the side effects and medical costs.Methotrexate is an important broad-spectrum anti-tumor drug and is frequently used in the treatment of treat liver cancer, lung cancer, breast cancer, ovarian cancer, gastric cancer, colorectal cancer and other kinds of cancers. According to the studies, the toxic and adverse effects of methotrexate are closely associated with677C>T and/or1298A> C polymorphism of the MTHFR (Methylene Tetrahydrofolate Reductase) gene. Hormonal drugs, letrozole, anastrozole and tamoxifen are mainly applied in the clinical treatment of breast cancer and ovarian cancer, and similarly, their therapeutic effects are associated with161T>G polymorphism of the CYP19A1gene and the CYP2D6*5allele. It is useful to genotype these related gene polymorphisms before the drugs are used, by which can facilitate drugs selection based on the detection results.There are plenty of methods for detecting gene polymorphism currently. In this study, we have established two assays: the first one is developed based on amplification refractory mutations system(ARMS) which is reliable, repeatable and easy-handling, aiming at detecting the three SNPs; the second one is developed for detecting the CYP2D6*5by relative quantitative realtime PCR. For each SNP, we have screened30samples, and then re-genotyping all samples using pyrosequencing technology as validation.100%accordance were observed between the results of these two methods, which in turn confirmed the performance of our established method. Compared with the pyrosequencing method, our established assays present many advantages.