Study On The Correlation And Mechanism Of Long - Chain Non - Coding RNA In Stress Response Of Endoplasmic Reticulum

Objective To explore the potential significance of long noncoding RNA (lncRNA) expressed in endoplasmic reticulum stress response also known as unfolded protein response (UPR).Method Mouse embryonic fibroblasts (MEFs) were isolated from 13 day mouse embryo, cultured and treated with tunicamycin for 4,8,12,16,24 hours to induce UPR. The activation of UPR was detected by PCR at these five time points. The untreated MEFs were used as negative control. The lncRNA expression profile was examined by a customized lncRNA array. The results of array assay were validated by real-time PCR and further analyzed by bioinformatics. The small-molecule inhibitors of UPR were used to investigate the lncRNAs associated with different arms of UPRResult There were 411 lncRNAs, whose expression levels were significantly up-regulated in MEFs treated with tunicamycin for 16 hours as compared with the controls, while 790 lncRNAs were significantly down-regulated. A number of significantly altered lncRNAs have been validated by Real-time PCR. A further bioinformatics analysis of lncRNA profile suggests that many could be involved in cellular metabolic processes and protein transport and localization. A long intergenic non-coding RNA named n416682 is highly enriched in the cytoplasm and its down-regulation seems to be associated with the PERK pathway.Conclusion Our lncRNA expression profile and bioinformatics studies suggest that many lncRNAs can either be regulated under UPR or could also in turn regulate UPR and other cellular processes.