Isolation And Identification Of Caffeoyl Quinic Acid From Solidago Canadensis And Tobacco

There are many bio active components in Solidago Canadensis, such as polyacetylenes, diterpenes, triterpenes, triterpenoid saponins, polyphenols, essential oils etc. There are many bioactive components in the tobacco, such as chlorogenic acid, nicotine, solanesol etc. These bioactive compounds can be used in agriculture, medicine and food industry. There are caffeoylquinic acids in both Solidago Canadensis and tobacco. Caffeoylquinic acids have many pharmacological effects, such as antibacteria, antiviral, anticancer etc. Solidago Canadensis and tobacco can be used as the resources to extract caffeoylquinic acids.Solidago Canadensis and tobacco, as the raw material, from the two lands of plants, the extraction, isolation and purification of caffeoylquinic acids are studied in this paper. The main achievement are as following:(1) The separation and purification of chlorogenic acid in tobacco was investigated. Through the research of the ultrasonic extraction, macroporous resin adsorption, polyamide column chromatography and ethyl acetate extract, the optimum conditions were determined.The extraction conditions were as follows:ethanol-water (40:60, V/V) used as extraction solvent, at50℃, extraction for1hour. Polarity macroporous resin CN-3can play the role of adsorption and decolorization to chlorogenic acid in tobacco extract. After the purification of macroporous resin, a column packed with polyamide was used to perform dynamic adsorption and desorption tests. The desorption conditions were as follows:ethanol-water (30:70, V/V) used as eluent, eluent volume4BV. Then ethyl acetate was used to extract chlorogenic acid in concentration and phase ratio was1:1(V/V), extracting2times. The purity of chlorogenic acid sample was more than85%.(2) The separation and purification of dicaffeoylquinic acid in Solidago Canadensis was mainly investigated. The optimum extraction solvent was ethanol-water (60:40, V/V). The content of chlogenic acid and dicaffeoylquinic acid in different parts of Solidago Canadensis was detected and the results showed that the content of chlogenic acid and dicaffeoylquinic acid both was the highest in the bud. Through the research of the solvent extraction, macroporous resin adsorption, polyamide column chromatography and ethyl acetate extract, the optimum conditions were determined. After petroleum ether decoloring, ethyl acetate was used to extract dicaffeoylquinic acid in water phase and dicaffeoylquinic acid was mainly concentrated in the ethyl acetate layer. After the ethyl acetate extraction, macroporous resin was uesed for further purification. The optimum parameters for the dynamic desorption process packed with the CN-5resin were as follows:after water elution to remove some impurities, ethanol-water (27:73, V/V) eluent and ethanol-water (45:55, V/V) eluent respectively. The dicaffeoylquinic acid was more than60%.(3) High performance liquid chromatography-mass spectrometry (HPLC-MS) was used to identify caffeoylquinic acids in five kinds of compositae plants and tobacco waste. Using this method, the high performance liquid chromatography (HPLC), total ion chromatography(TIC) in negative ion monitoring mode, MS and MS2spectrum of these plants were detected. By analyzing TIC characteristics and MS fragmentation patterns,7caffeoylquinic acids were assigned to be two categories, four mono-caffeoylquinic acids, three dicaffeoylquinic acids.In addition, chicoric acid, a caffeic acid derivative, was identified in this work.