Study On The Design, Synthesis And Bio - In Vivo Imaging Of Fluorescent Probes Based On Lysosomes And Nucleotides

Life is made of cells, during the process of cell differentiation, metabolism and aging, nucleotide is everywhere. Nucleotide can improve the body’s functions, strengthen the organs of an autoregulatory capacity. Probes can be used to locate the small molecules in cells, redox reactions in cells, cell division and apoptosis, and can also detect he fusion process between virus and cells, analysis of molecular structure on the surface of the cell membrane properties, and so on. A probe which can locate both the micromolecule and organelle is necessary for our study.In order to effectively detect the biomolecules and the organelle, our study in thesis were summarized as follows:1. We developed a lysosome-targeted fluorescence chemodosimeter,1, for monitoring the endogenous and exogenous H2S by in-vivo imaging study. Compared to the commercial tracers,1was found to be an excellent lysosomal stain for the in-vivo imaging using HeLa cells and C. elegans to trace the location of exogenous H2S with high Pearson’s and overlap coefficients. In particular,1is the first disulfide-bond-containing coumarin derivative to trace the accumulation of lysosome in D. melanogaster and endogenous lysosomal injury in mutated C. elegans (SRP-6nulls) with a high resolution.We develop a new1,8-naphthalimide-based fluorescence sensor (NUH1) for monitoring the uridine diphosphate (UDP). NUH1with uridine of nucleotides combination through the hydrogen bonding and exhibited a fluorescence enhancement associated with formation of a complex with UDP. The results indicate that NUH1is a highly selective and sensitive sensor for UDP. NUH1can be applied to image UDP in living cells and also an excellent stain for the in-vivo imaging using HeLa cells and C. elegans to trace the UDP.