| Pu-er tea was made from the leaves of Camellia sinensis. Owing to its special characteristic, pharmacologic properties and beneficial result, Pu-er tea had became more and more popular in China. This paper mainly reported the chemical compounds were isolated form pu-er tea and biological properties. With the combined chromatographic methods and techniques,12 compounds were isolated and identified on the basis of NMR, MS spectra as 1,1’-(oxydi-didihydroconiferyl alcohol) (1), caffeine (2), kaempferol (3), trans-p-coumaric acid (4), phloretic acid (5), quercetin (6), methyl-β-D-glucopyranoside (7), P-hydroxybenzoic acid(8),3,4,8,9,10-pentahydroxy-dibenzo[b,d]pyran-6-one (9),5,7-dihydroxycoumarin (10),1,3,8-trihydroxynaphth-alene (11), myricetin (12). Compound 1 was obtained as natural product for the first time. Kaempferol (3) inhibited the growth of Staphylococcus aureus with MIC of 64 μg/mL.In addition, endophytic fungal isolates, Aspergillus sp.Y-12 and Trichoderma koningiopsis YIM PH30002 were employed for chemical investigation and bioactiveassay.With the combined chromatographic methods and techniques,10 compounds were isolated from Aspergillus sp.Y-12. Their structure were identified by the data of NMR, MS as asperginsin A (1), (R)-10,11-dihydroxyfarnesol (2), sterigmatocystin (3), 4-hydroxy-5-methoxy-benzoic acid (4), microperfuranone (5), ergosterol endoperoxide (6), octadecanoic acid (7), canangalias A (8),6-octadecenic acid (9), 4,8-dimethylnona-3,7-dien-l-ol (10). Compound 1 was elucidated as a new compound, compound 2 were isolated as a natural product for the first time, Asperginsin A (1) inhibited the growth of Alternaria tenuissima PH30016 with MIC of 64μg/mL. Moreover, some compounds showed synergistic antimicrobial activity.2 compounds were isolated from Trichoderma koningiopsis YIM PH30002 and identified as 7-O-Methylkoninginin D (1),7-O-acetyl-koninginin D (2). Compound 2 was elucidated as a new compound with certain antibacterial activity. |
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