Detection Of MilRNAs 764,799 Gene Knockout And Its Target Gene And Function In

Arthrobotrys oligospora is the firstly reported nematode-trapping fungus that can develop adhesive networks to capture nematodes, which makes it a model to study the interactions between nematode-trapping fungi and their nematode preys. In this study, we knocked out two microRNA-like RNAs (milRNAs) and studied their functions in development of trapping devices. In addition, the target genes of the two milRNAs were predicted.The yeast cells were prepared for transformation by electroporation and PEG/CaC12-mediated transformation method was used for obtaining protoplasts. The two milRNAs deletion strains were obtatined and compared with the wild strains in different phenotypes, including sporation, colony growth, induction of adhesive networks, and tolerance to growth stress. The results showed no significant difference between the deletion and wild strains except that the milRNA799-deletion strain developed less trapping cells on induction of urea.RNAhybrid, pita, Miranda, targetscan and RNA22 were used to predict the target genes of the two milRNAs and the InterProScan tool was used to annotate the putative target genes.There are two main characteristics in this paper.1. Two milRNAs were knocked out in Arthrobotrys oligospora, which was compared with the wild stains in various phenotypes to study their functions.2. Target genes of the two milRNAs were predicted, as well as their putative functions.