For Copper, Zinc, Chromium And Other Metal Ion Detection Of Fluorescent Probe Molecules Synthesis

Fluorescent probe recognition, as a commonly used analytical method, has many properties such as good selectivity, high sensitivity, simple and convenient and so on and it has been developed quickly in recent years. Currently, the application of fluorescent chemical probes of Zn2+ and Cu2+ and other metal ions is still subject to some restrictions. For example, some of them are lack of specificity and are susceptible to interference from other metal ions, some of them are poorly water-soluble which must be used in the organic solvents or organic solvent and water mixed solvents, some of them are complicated and are difficulty to synthesis, some of them are poor of membrane permeability, some of their excitation wavelength are in the ultraviolet region, which is restricted in biological detection. Therefore, it has great theoretical and practical significance to optimize the design and synthesis of the fluorescent chemical probe molecule such as Zn2+ and Cu2+ and to search for fluorescent chemical probes that have good selectivity, high sensitivity, long excitation wavelength, good water solubility and superior performance.This thesis has designed and synthesized a series of fluorescence 2-pyrazoline, indolizine and derivatives of cyclazines, studied fluorescence properties of these compounds, and investigated their applications in recognition of Zn2+ and Cu2+.1. A series of derivatives of schiff base (3、6) were synthesized and their optical properties were tested. Their applications in molecular recognition were investigated as well. The results indicated that derivatives of schiff base (3、6) had a recognition toward chromium ion,but they were easily disturbed by other ions.3 also had a selective recognition toward copper ion. The fluorescence intensity of 2a had a great change in a certain pH range.2. A derivative of quaternary ammonium salt 4 was synthesized and their optical properties were tested. The UV-Vis spectrum of these compounds and fluorescence spectrum in the solutions of different pH were investigated. Their applications in recognition toward transition metal ions were preliminarily investigated. The results showed that 4 had fluorescence response toward zinc ion, cobalt ion and copper ion, especially with high sensitivity toward copper ion, but it was easily disturbed by other ions. The result provides new experiment content for the design of novel copper ion fluorescence probe.3. Derivatives of heterocyclic compunds modified by salicylaldehyde were synthesized and their optical properties were tested.Their applications in recognition toward transition metal ions were preliminarily investigated as well. The fluorescence intensity of compound 5a reduced toward copper ion、chrome ion、cobalt ion, while it increased toward zinc ion and mercury ion.5a had a distinct recognition toward zinc ion,but it was easily disturbed by mercury ion. The fluorescence intensity of compound 4b reduced greatly toward zinc ion, but it was easily disturbed by copper ion. The result indicated that the fluorescence intensity of 4a had a great change in a certain pH range.4. Derivatives of heterocyclic compunds modified by ehylenediamine were synthesized and their optical properties were tested. The fluorescence spectrum in the solutions of different pH were investigated. Their applications in recognition toward transition metal ions were preliminarily investigated as well. The fluorescence intensity of 5a had a great change in a certain pH range. The result provides new experiment content for the design of novel hydroxide ion fluorescence probe. The fluorescence intensity of compound 6a enhanced greatly toward chrome ion. So the result provides new experiment content for the design of novel chrome ion fluorescence probe.