The Biodegradation Of Chloroguaiacol And The Inhibitory Effect Of Lignosulfonate On Methanogen

Pulp wastewater contains large amounts of chlorinated organic pollutants, most of them could lead to carcinogenic, mutagenesis teratogenesis and other hazards. The chloroguaiacol is the major toxic compounds in pulp wastewater, which produced in the process of pulp bleaching stage.Due to the serious environmental pollution by pulp wastewater, so the study of removal toxic pollutants from pulp wastewater become a research key point in recently years.In this paper, we mainly use the method of microbiological, select the microorganisms which isolated from the sludge of pulp wastewater,use the single substituted chloroguaiacol (4-chloroguaiacol) as the target for biodegradation experiments.The results showed that the strains ZD 8 which isolated from the sludge of pulp wastewater has certain biodegrability, under the conditions of 30℃, pH6,160 rpm,it could degraded the 4-chloroguaiacol in the concentration of 40mg/l, and discussed the effect of add co-substrates in the degradation of 4-chloroguaiacol.In addition, the experiment were employed to study the inhibitory levels of the lignin on acetate-utilizing methanogens. Lignosulfonate showed inhibition of the methanogenesis expressed as a long lag times. The methanogenesis under thermophilic (50℃) conditions was more susceptible to the inhibitory effects of lignosulfonate than mesophilic conditions (40℃and 30℃). Moreover, increaseing in the anaerobic digester sludge concentration decreased the inhibitory effects of the softwood lignin.