Ni & Lt; 3 & Gt; C And Fe ₂ 2 Nanoparticles In Vivo And In Vitro

Cancer is also Malignant tumor, damaging the structure and function of tissues and organs which is becoming one of the main reasons for the current death. Chemotherapy and radiotherapy are mainly method to treat cancer. However they can cause negative influence to normal tissues. Therefore it is urgent to find new treatment instead of traditional method. Hyperthermia is using the biological effects of heat to treat cancer, which is certified as following surgery, radiotherapy, chemotherapy, biological therapy the fifth largest after treatment by the U.S. FDA (Food and Drug Administration) in1985. Developing new materials of hyperthermia has become an important task in clinical treatment of cancer now. This article dedicates to the research of new and biocompatiblc nanomaterials as hyperthermia agents. We explore the Ni3C and Fe5C2nanoparticlcs (NPs) as hyperthermia materials, and have made some meaningful results.In part one, Ni3C NPs is synthesized with iron acetylacetonate and nickel acetylacetonate precursor which is added to olcylamine and octadecene by thermal decomposition method with the diameter around diameter of50nm. Ligand exchange method is adopted to improve the solubility by PEG-COOH. Ni3C NPs exhibits a broad absorption from UV to near-infrared region (400-1000nm), which results in the rapid rise in temperature irradiated by an808nm laser. In vitro and in vivo cytotoxicily demonstrate Ni3C NPS is good biocompatibility. Moreever, treatment experiments of HeLa cells and in tumor mice proved that Ni3C is a potential photothermal material.In the second part on the basis of NijC NPS developing Fe5C2as hyperthermia agent, which is prepared by high temperature pyrolysis with the diameter of13nm. Fe5C2NPS is modified to become water-soluble with Phospholipid material. The UV absorption spectra indicates Fe5C2nanoparticles in a broad range of400-1000nm absorption. When using808nm laser with power density of1.5W/cm2, the Fe5C2, NPS aqueous solution (0.5mg/mL) can raise20.2℃. Fe5C2NPs saturation magnetization is33.1Am2/Kg, which can be used as a T2contrast agent. In the magnetic field strength of60.6KA/m (200KHz), the concentration of2mg/mL Fe5C2NPS solution temperature raises19℃, which is proving as potential magnetic hyperthermia material. In vivo therapeutic experimental effect in tumor mice is a good description that Fe5C2NPs is dual function material with photothermal and magnetic hyperthermia effect.