Study Of The Colonization And Gene Expression On Arthrobacter Sp.DNS10 In Black Soil

Atrazine is the most widely applied in Northeastern Black Earth region of herbicide made in recent years, which resulted in a large number of the irrational administration asked a series of environmental, ecological security and endanger human health. In this study, four locations in Black Earth Region(A’cheng, Changchun, Changtu, Shenyang) of maize soil as test soil to pre-isolated laboratory efficiency atrazine degrading bacteria Arthrobacter sp.DNS 10 for the study object, the DNS 10 in these soils colonization, gene expression and different soil environment, the actual degradation under different degrees of contamination in. The results are as follows:In the simulation study of a one-month restoration, the natural degradation of atrazine in the soil are able to different regions in a certain degree, the study adding degrading bacteria, the degrading bacteria can even accelerate effective given atrazine in the soil Tianjin degradation.Studies exploring the use of technical analysis TRFLP atrazine degrading bacteria Arthrobacter sp.DNS 10 colonization in the soil. The results showed that the DNS 10 different environments, both have a high ability to adapt to rapidly collect contaminated soil in the breeding to accelerate the removal of atrazine residues in the soil. In the first five days of repair, DNS 10 reproduce quickly, which is capable of atrazine as the sole source of nitrogen for growth.The study also tries to apply real-time PCR technology, the degrading bacteria DNS 10 gene expression in different soils. The study found that in a simulated Restoration of 30 days, the degradation of gene copy number was significantly lower overall trend. On day 5, gene copy number rapidly decreased after inoculation. Late rate of decline slowed. Biodegradation of atrazine in the early stage of atrazine degradation due speed, a lot of atrazine degradation by soil microorganisms after the lack of nitrogen source necessary for the growth, the number of rapid reduction.At the same time, the study found that the test soil Shenyang extracted due to the relatively low organic matter content, atrazine receives less organic matter in the soil, so the soil degrading bacteria DNS 10 in Shenyang area The reproductive capacity and gene expression were significantly higher than the other three regions of the soil samples. In the four regions, the soil sample Changchun high organic matter content, degrading bacteria where atrazine degradation rate is also the slowest. Visible, we have the opportunity to really have atrazine degradation caused impediment, but its specific mechanism of action more in-depth follow-up study has yet to be.Based on these studies, we choose the most suitable breeding atrazine and play their effectiveness tested soil degradation, such as grouping applying different concentrations of atrazine, different concentrations of atrazine degradation of bacteria DNS10 colonization, gene expression what kind of impact.The study found that atrazine degrading bacteria DNS10 atrazine in the environment pollution has a strong tolerance to higher pollution concentrations in the soil can still grow better and play their degradation effect. But high concentrations of atrazine contamination, degradation Degradation gene expression will have an impact, therefore, long-term exposure to high concentrations of atrazine pollution of the environment, for the degrading bacteria DNS10 normal growth and degradation of contaminants may It will have some impact.