| Antibiotics bacteria residue is a potential risk to environmental, which was listed as the national list of hazardous waste in 2008. ABR must be disposed as hazardous waste, so the safe disposal of ABR has become a problem restricting the development of the pharmaceutical industry at present. This research mainly focused on penicillin bacterial residues(PBR). Firstly, the HPLC-MS/MS method was adapted to measure residual penicillin G in antibiotics bacteria residue. Combining the characteristics analysis, the effect of alkaline-thermal pretreatment method on penicillin bacterial residues were investigated to improve the dissolution rate of intracellular substances and then confirmed the optimal pretreatment conditions. Then the anaerobic digestion of the treated and untreated penicillin bacterial residues was carried out to determine the optimal control parameters. Lastly, the mix anaerobic digestion easing the inhibition of ammonia nitrogen concentration with PBR and food waste was adopted to determine the optimum C/N ratio. The research results were summarized as follows:1) The method, which based on solid phase extraction-ultra fast liquid chromatography- tandem mass spectrometry(HPLC/MS/MS), was developed to determine penicillin in the PBR. Firstly, the samples were purified through protein precipitation process with 20% zinc sulfate and 10% potassium ferrocyanide. Then, the tetracyclines was enriched and purified with Oasis, separated through Agilent Plus C18 using 0.1% formic acid solution and acetonitrile as mobile phase. Lastly, the tetracycline was detected by mass spectrometryin positive ion mode with multiple reactions monitoring using an electrospray ionization interface. The detection limit(S/N=3) was 1.5ng/mL, and the linear range was 1.5 to 100 ng/mL, with a correlation coefficient 0.9995. The method results are accurate, high recovery rate, and good reproducibility. The penicillin in PBR was determined to 2000-6000 mg/kg via the detecting method.2) By the biochemical methane potential(BMP), the optimal control parameter was confirmed as alkaline-thermal pretreatment. And the optimum parameters was temperature 70°C, the reaction time of 2.0 h, alkali concentration of 2%, and moisture content of 90%. Under the optimal pretreatment conditions, the concentration of SCOD enhanced from 3414.12 to 9863.25 mg/L, the COD dissolution rate was 72%, and the methane production rate increasesed from 0.19 to 0.24LCH4/gVS, which was enhanced by 26.3% more than the untreated PBR. The residual penicillin removal rate can reach more than 99.99%. Concentration of penicillin residue is less than 400 mg/kg residue.3) The results of anaerobic digestion of the untreated PBR shows that organic loading rate(OLR) was 3.98gVS/(L?d), biogas yield was 4.21L/d, biogas production rate was 0.35 L/(gVSadded?d), and COD removal rate is 80.93%, respectively. The results also show that OLR, biogas production rate, and the COD removal rate were 5.05 g VS/(L?d), 0.42L/(gVSadded?d), and 91.92%, respectively, which was enhanced by 20%, 20%, and 13.5% more than the unreated PBR, respectively. But the concentration of ammonia nitrogen was 3500 mg/L, which restrained anaerobic digestion in some degree.4) After the the co-digestion, the concentration of ammonia nitrogen of the anaerobic alone of PBR alone was 3186 mg/L. The biogas production and biogas production rate in the carbon-nitrogen ratio of 25/1 was 5.81 L and 0.38L/(gVSadded?d), respectively, which was enhanced by 35.71% and 11.76% more than the unreated PBR, respectively. The average concentration of ammonia nitrogen was 2617.82mg/L, which was decreased by 17.85% than that of the untreated PBR. The PBR and food waste were 26.83 g and 128.53mL(C/N=25/1) as the best dosage carbon-nitrogen ratio. |
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