| Chapter 1:This chapter presents the supramolecular chemistry, molecular recognition and fluorescent probe. The concept, composition, recognition mechanism and category of fluorescent probe were briefly introduced. In addition, the recent research progress of coumarin-based fluorescent probe was introduced. Finally, research situation of fluorescent probe for selective detection of HSO3-, Trp and Fe3+ were also recommended.Chapter 2:A coumarin-based dye, Fluorescent Red GK, was developed for the detection of bisulfite anion. The probe has exhibited high selectivity and sensitivity against other anions at room temperature. Upon addition of HSO3-, the strong fluorescence of Fluorescent Red GK was severely quenched and its color changed significantly from orange to colorless under illumination with a UV lamp. The color of the solution was also changed from pink to colorless. As a result, it can be used as a specific colorimetric and fluorescent probe for bisulfite anion. In addition, the detection limit was as low as 1.76μM. The results showed that the Fluorescent Red GK could be used in the design of the fluorescent probe for detecting HSO3- in aqueous solution. Furthermore, the probe has been used for determination of bisulfite in sugar samples with satisfactory results.Chapter 3:A novel coumarin derivative,4-formyl-7-hydroxylcoumarin (FHC), was developed as a fluorescent probe for tryptophan. The derivative effectively recognized tryptophan at neutral pH using UV-Visible spectra and fluorescent spectrophotometry. A dramatic increase in fluorescence was observed in the presence of tryptophan. FHC exhibited high selectivity and sensitivity when used with other amino acids at room temperature. Confocal fluorescent imaging with FHC demonstrated the probe could detect intracellular tryptophan in living cells. The detection limit of tryptophan with FHC was 1.65 μM.4-formyl-7-hydroxylcoumarin allowed the rapid, selective and sensitive detection of tryptophan in 1:1 CH3CN/HEPES media.Chapter 4:A coumarin derivative,7-(Diethylamino)-2-oxo-2H- chromene-4-carbaldehyde, was developed for the detection of Fe3+ The probe has exhibited high selectivity and sensitivity against other metal ions at room temperature. Upon addition of Fe3+, the strong fluorescence of 7-(Diethylamino)-2-oxo-2H-chromene-4-carbaldehyde was severely quenched under illumination with a UV lamp. As a result, it can be used as a specific fluorescent probe for Fe3+. In addition, the detection limit was as low as 0.217nM. The results showed that the 7-(Diethylamino)-2-oxo-2H-chromene-4-carbaldehyde could be used in the design of the fluorescent probe for detecting Fe3+ in aqueous solution. Confocal fluorescent imaging with the probe demonstrated the probe could detect intracellular Fe3+ in living cells. |
