(Z) -2 - Nitro - Dibenzo [b, D] Oxa - 7 (6H) - Ketoxime In HepG2 Cells

The effect of (Z)-2-nitrodibenzo [b,d]oxepin-7(6H)-one oxime (NOO) on human liver cancer HepG2 cells was investigated in this paper. Thirty-six new synthesis of biphenyl compounds were detected the cytotoxic effect through MTT method, in all the compounds that were tested, IC50 value of NOO is the lowest. At the same time, we judge preliminary cell apoptosis by fluorescent staining and cell cloning, we analyze the mechanism of inducing tumor cell apoptosis by DNA gel electrophoresis, immunoblotting and mitochondrial membrane potential. To some extent, what NOO inhibited the proliferation of HepG2 cells has a certain relationship that time-and dose-dependent manner. Fluorescence staining and DNA gel electrophoresis results showed that the cells produce the apoptotic bodies and DNA fragmentation by NOO, and indicated NOO can induce HepG2 cells apoptosis. Flow cytometry instrument testing showed that NOO can lead to mitochondrial membrane potential losses; The protein imprinting results showed that procaspase 3, procaspase 9 and the Bcl-2 protein expression decreased,it instructs caspase-3 and caspase-9 protein is activated. NAC can inhibit (Z)-2-nitrodibenzo[b,d]oxepin-7(6H)-one oxime in HepG2 cells cancer cell apoptosis. Theresult shows that cell oxidative damage and activate the ROS releases. Therefore, (Z)-2-nitrodibenzo [b,d]oxepin-7(6H)-one oxime induced cell apoptosis by the mitochondrial, (Z)-2-nitrodibenzo[b,d]oxepin-7(6H)-one oxime may be used the treatment of tumor diseases.